Why Do Beets Have The Ability To Dissolve Cell Membranes
In this laboratory experiment is stating that organic lipids have the ability to dissolve lipids membranes, and in this exercise acetone would be the main source to be used to have the reaction of dissolving the lipids inside the tubes.
This exercise was to be able to observe how the chemicals reacted in rare temperatures such in heat and in cold weather, also this type of changes can create a new color either making the color lighter or darker. The reaction of a light color would be that it did not affected the beet for the fact that the chemicals that were inserted In the tubes were not strong in the other hand the tubes that had darker colors, would be that the chemicals were strong enough to damage the beetle.
In this experiment their was two types of hypothesis. The null of the hypothesis is that, if the beets are exposed to acetone, the reaction right away could be overserved that their was no physical or chemical effect on the tonoplast membrane.
The alternative hypothesis in this laboratory experiment, was that if the beets were exposed to acetone, the tonoplast membrane will be able to release betacyanin a red dark color. …show more content…
The first steps for this laboratory exercise was to follow all laboratory safety procedures, such as putting gloves and avoid any hazard materials that needed attention.
To begin the experiment they needed to be 13 clean tubes available to continue the lab, however one tube would be put aside for further instructions, the 12 tubes were separated into 4 groups making each group have 3 tubes. In the first group tubes 1 through 3, DI Water was inserted in the tube and 7mL of 0% Acetone. The second group of tubes 4 through 6, 7 mL of 4% Acetone were introduced in the tubes. The third group of tubes 7-9, 7 mL of 9% Acetone were introduced. In the fourth group of tubes 10 through 12, 7 mL of 20% Acetone were
introduced.
The timing on this experiment was from 0 min to 20 min, every 5 min the tubes were placed in either hot water or cold weather. This would be able to make a chemical reaction making the colors go lighter or darker, to be able to see the differences of the color changing a label of 1 through 10, to be specific the lower the number the lighter the color was and the higher the number the darker it would be. However some reactions could be able to stay the same even if the solvents were combined. The used of the quantitative method table, would be to check and record the absorbance at 460nm on the spectrometer.
The testing in this laboratory exercise, is because the beets contained betacyanin in the roots. In this laboratory, Ms. Mendez stated to observe how much qualitative and quantitative data could we get from the chemical reactions that were about to be exposed. However the alternative hypothesis was correct, because as the time went by the beets started releasing a reddish dark color. This was observe after the beets were introduced to hot temperatures, making the chemical reaction and damaging the walls of the membrane. Cold temperatures however, started creating water to crystalize, making them go to a bigger size, because of respective hydrogen bonds in the permeable membranes.
In this experiment they were only 3 concentration of acetone in total the first one was 4% Acetone, the second one 9% Acetone, the third one 20% Acetone, also Distilled water (DL H20) was also use. The experimental observation was to conclude which chemicals made the most damage and which did not. However the idea was that if the tubes had 20% Acetone it would create more damage.
When obtaining the beets, a cork with 5mm inside diameter were used to be able to extract them in a precise measurement, to be able to insert the beets inside the tubes with out making any mess. Before inserting the beets in the tubes, they were rinsed with tap water for 2 minutes, to be able not to have damage cells in the surface of the tubes. After placing the beets in each label group of tubes, than were placed on respective temperature treatments to begin the chemical reaction if do so.
This exercise was to be able to observe how the chemicals reacted in rare temperatures such in heat and in cold weather, also this type of changes can create a new color either making the color lighter or darker. The reaction of a light color would be that it did not affected the beet for the fact that the chemicals that were inserted In the tubes were not strong in the other hand the tubes that had darker colors, would be that the chemicals were strong enough to damage the beetle.
In this experiment their was two types of hypothesis. The null of the hypothesis is that, if the beets are exposed to acetone, the reaction right away could be overserved that their was no physical or chemical effect on the tonoplast membrane.
The alternative hypothesis in this laboratory experiment, was that if the beets were exposed to acetone, the tonoplast membrane will be able to release betacyanin a red dark color. …show more content…
The first steps for this laboratory exercise was to follow all laboratory safety procedures, such as putting gloves and avoid any hazard materials that needed attention.
To begin the experiment they needed to be 13 clean tubes available to continue the lab, however one tube would be put aside for further instructions, the 12 tubes were separated into 4 groups making each group have 3 tubes. In the first group tubes 1 through 3, DI Water was inserted in the tube and 7mL of 0% Acetone. The second group of tubes 4 through 6, 7 mL of 4% Acetone were introduced in the tubes. The third group of tubes 7-9, 7 mL of 9% Acetone were introduced. In the fourth group of tubes 10 through 12, 7 mL of 20% Acetone were
introduced.
The timing on this experiment was from 0 min to 20 min, every 5 min the tubes were placed in either hot water or cold weather. This would be able to make a chemical reaction making the colors go lighter or darker, to be able to see the differences of the color changing a label of 1 through 10, to be specific the lower the number the lighter the color was and the higher the number the darker it would be. However some reactions could be able to stay the same even if the solvents were combined. The used of the quantitative method table, would be to check and record the absorbance at 460nm on the spectrometer.
The testing in this laboratory exercise, is because the beets contained betacyanin in the roots. In this laboratory, Ms. Mendez stated to observe how much qualitative and quantitative data could we get from the chemical reactions that were about to be exposed. However the alternative hypothesis was correct, because as the time went by the beets started releasing a reddish dark color. This was observe after the beets were introduced to hot temperatures, making the chemical reaction and damaging the walls of the membrane. Cold temperatures however, started creating water to crystalize, making them go to a bigger size, because of respective hydrogen bonds in the permeable membranes.
In this experiment they were only 3 concentration of acetone in total the first one was 4% Acetone, the second one 9% Acetone, the third one 20% Acetone, also Distilled water (DL H20) was also use. The experimental observation was to conclude which chemicals made the most damage and which did not. However the idea was that if the tubes had 20% Acetone it would create more damage.
When obtaining the beets, a cork with 5mm inside diameter were used to be able to extract them in a precise measurement, to be able to insert the beets inside the tubes with out making any mess. Before inserting the beets in the tubes, they were rinsed with tap water for 2 minutes, to be able not to have damage cells in the surface of the tubes. After placing the beets in each label group of tubes, than were placed on respective temperature treatments to begin the chemical reaction if do so.