In the test tube 4, 5, 6 that contained yeasts and water only, the yeast did not have food which was sugar. That was the reason why test tube 4, 5 and 6 did not expand. In the test tube 1, 2, 3 which contain yeast, water, and sugar, the yeast should have thrived and made a lot of carbon dioxide, so that the balloon expand bigger and bigger
The reason we use three test tubes with yeast, sugar, and water and three test tubes with just yeast and water, instead of only one test tube with each type of mixture is because we had to compare the difference in size between each balloon and clarify that each of the test tubes that contain sugar will expand.
In this experiment, we had grown yeast in the test tube filled with water and sealed with a balloon. These growth conditions were considered as anaerobic because they were sealed and the amount of oxygen in the test tube will be reduced in a short time. Thus, these yeasts will carry out anaerobic