You Light Up My Life Lab
Title: You Light Up My Life
Purpose: To determine how different environmental conditions affect a plants starch levels.
Hypotheses:
1. If the plant that is in absence of CO2 and is kept in a closed environment, then it will have a starch level of 1 because the plant is missing CO2, which it needs to complete the photosynthesis process.
2. If the plant is in a closed environment, then the plant will have a starch level of 3 because it has all the environmental conditions it needs.
3. If the plant is kept in a dark environment, then it will have a starch level of 1 because it’s missing a fundamental resource needed for photosynthesis.
4. If the plant is kept in the presence of light,air,soil,and water, then the plant will have a starch level of 3 because it has all of the necessary environmental conditions.
Materials: safety goggles iodine solution petri dishes razor blade forceps beakers trays potting soil plastic lids
95% ethyl alcohol sodium hydroxide medicine cups
Procedures:
1. Compare the plants in each tray(characteristics)
2. Label petri dishes dark, closed, normal, closed no CO2
3. Take one leaf from a plant in each try
4. Immerse leaf in boiling water for 1 minute
5. Remove the pigments by immersing each leaf in hot 95% ethyl alcohol
6. When most of the pigment is removed, remove the leaf from the hot alcohol; dip each leaf in hot water again for a few seconds
7. Place each leaf in the petri dish and cover it with 3 drops of iodine solution. Allow leaf to sit for a few minutes.
8. Remove the leaf and dip into a beaker of clean water to rinse it. Pour iodine into designated iodine waste. Rinse petri dish.
9. Place leaf back in the petri dish.
10. Repeat steps 4-9 for all conditions
Variables:
Manipulated: absence of light, absence of CO2, closed environment
Responding: If starch is present, according to leaf color
Controlled: amount of iodine, amount of water, same type of radish seeds, same type of soil, time in boiling water, amount of alcohol
Purpose: To determine how different environmental conditions affect a plants starch levels.
Hypotheses:
1. If the plant that is in absence of CO2 and is kept in a closed environment, then it will have a starch level of 1 because the plant is missing CO2, which it needs to complete the photosynthesis process.
2. If the plant is in a closed environment, then the plant will have a starch level of 3 because it has all the environmental conditions it needs.
3. If the plant is kept in a dark environment, then it will have a starch level of 1 because it’s missing a fundamental resource needed for photosynthesis.
4. If the plant is kept in the presence of light,air,soil,and water, then the plant will have a starch level of 3 because it has all of the necessary environmental conditions.
Materials: safety goggles iodine solution petri dishes razor blade forceps beakers trays potting soil plastic lids
95% ethyl alcohol sodium hydroxide medicine cups
Procedures:
1. Compare the plants in each tray(characteristics)
2. Label petri dishes dark, closed, normal, closed no CO2
3. Take one leaf from a plant in each try
4. Immerse leaf in boiling water for 1 minute
5. Remove the pigments by immersing each leaf in hot 95% ethyl alcohol
6. When most of the pigment is removed, remove the leaf from the hot alcohol; dip each leaf in hot water again for a few seconds
7. Place each leaf in the petri dish and cover it with 3 drops of iodine solution. Allow leaf to sit for a few minutes.
8. Remove the leaf and dip into a beaker of clean water to rinse it. Pour iodine into designated iodine waste. Rinse petri dish.
9. Place leaf back in the petri dish.
10. Repeat steps 4-9 for all conditions
Variables:
Manipulated: absence of light, absence of CO2, closed environment
Responding: If starch is present, according to leaf color
Controlled: amount of iodine, amount of water, same type of radish seeds, same type of soil, time in boiling water, amount of alcohol