The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by spectrophotometric determination of NADH at 340 nm. From Pierce BCA assay of crude homogenate, initial protein concentration was shown to be 100 mg/ml. The final protein concentration of the pooled affinity sample was shown to be 0.2 mg/ml. It was found that the total specific activity of LDH was 58.5 µmol/min/mg, and yield of 0.6%. Even though we were successful in purifying LDH enzyme, further steps can be taken to increase the yield.…
The story told in Slaughterhouse Five is very much unique to its setting and the time in which it occurs. The story is told by Kurt Vonnegut, who is also a minor character in the book, about the life experiences of Billy Pilgrim during World War II. The story centers on a specific event that occurs during the war, the Allied firebombing of Dresden, Germany. This specific bombing has gone virtually unnoticed by Americans throughout history since the war due to its location, being in Germany. However, certainly the scrutiny would be much greater if such a horrendous bombing was inflicted upon Britain, America, or another Allied power. In addition, the story itself would not have been told if it were not for Vonnegut’s own unique situation…
Handguns comprised 72.5 percent of the firearms used in murder and non-negligent manslaughter incidents in 2011; 4.1 percent were with shotguns; 3.8 percent were with rifles; 18.5 percent were with unspecified firearms.…
The vast amount of causes for conflict and the ambiguity of each reaches an overwhelming total. The sort of a particular conflict is highly dependent on why the conflict has occurred, as individuals posses differing core values and beliefs. Conflict is born when aspects of individuals or groups clash. There are infinite aspects that make up an individual, but three remain prominent when it comes to tracing where conflict has originated from. Ideas, interests, and hopes or expectations of an individual are all integral factors that can lead to both small and large scale conflict.…
Conflict is omnipresent for every individual regardless of time and place. It is a fundamental part of human existence, leading back to the earliest forms of conflict where our human ancestors competed with other species for survival tools such as food and shelter. Nowadays, conflict has developed into multitudinous forms – from merely debating what to have for lunch to global issues such as climate change. Without conflict, our experiences in life would undoubtedly be less dynamic and vibrant. Conflict is an essential factor to shaping our identity. Our relationship with conflict defines who we are because we learn to formulate our own opinions and values. Conflict is a natural element in the world and although we may not notice each and every single time it presents itself, conflict is a part of our everyday lives.…
Conflict is an inevitable part of life whether it is in relationships, socially, or between nations. Most conflicts arise from differing points of view on actions or goals and how they are handled can lead to a ceasefire or the breakup of one’s relationships. Conflict resolution skills are needed when navigating the stormy waters of conflict. How one perceives the goals and intentions of others can lead to misconceptions. When an individual or group perceives another’s intentions are harmful conflict will arise. It is human nature to believe that one’s group is different or better than another group. Forming an “in-group bias” will lead one to look for evidence to support one’s negative opinions of others. Stereotyping is often behind negative opinions and misconceptions that lead to conflicts.…
In the award winning movie, Ordinary People, Calvin, Beth, and Conrad face major conflict due to silence and violence to the loss of their family member, Buck. The family endures many feelings to discuss but they are avoiding communication. Conrad is the main character and struggles with the loss of his brother. This affects him deeply as well as the family and causes avoidance of one another. They easily fall into arguments and quit many daily activities due to grief. Although the family suffered a major loss, it leads to major silence and violence, causing a lot of conflict in the movie.…
When confronted with conflict, peoples moral centre can cause them to act in heroic ways. When we are exposed to war and devastation our reactions to this......... Thousands of Chinese students gathered in a peaceful protest for basic Democratic reform at Tiannaman Square 1984. Due to these actions they were senselessly and inhumanely killed in cold blood by the Chinese Military. The students were unarmed and in no way of a danger to anyone. This devastation shocked the world and erupted massive conflict. One thing that this devastation proved to the world that during times of conflict where ones moral compass is tested there true heroism is revealed. A small, unexceptional figure in pants and white shirt, looking tiny next to the hulking tanks, carrying what looks to be his shopping, positions himself before an approaching tank, with a line of 17 more tanks behind it. The tank swerves right, he moves in front again. The tank swerves left, he moved in front again. Then this anonymous bystander climbs up onto the vehicle of war and says something to its driver, "Why are you here? My city is in chaos because of you." With a single act of defiance, a lone Chinese hero revived the world's image of courage. He was the “unknown rebel” also know as the Tanks Man a man who was immensely courageous and a beacon of hope. The courage that it took to walk out against such massive machinery, knowing good and well that it could very well be your last day on this earth is inspiring. He took courage to a whole new level, becoming a world hero of sorts. This ordinary everyday man had stood up to one of largest of armies which was an undeniably, extraordinary act. Another man who stood up for what he believed in response to such brutal and callous acts was a man named Bradley Manning. A man who new his moral obligation was to show the world what the Government was hiding from them. Manning was an…
I am writing in response to the prompt ‘conflict is an inherit part of life; it isn’t necessarily negative. Based on my knowledge of the text, The Rugmaker of Mazar e Sharif I will write an expository piece in the form of an analytical essay to highlight to the readers that conflict isn’t necessarily negative. I have chosen to do this because I believe that conflict is a part of life as it can bring good and bad for individuals. The target audience for my discussion is young people, 16-18 years old, who are studying the text and keen to engage with ideas of conflict.…
Conflict, whether we choose to admit it, is a part of life. Conflict may be sparse in a persons life, and most of which is over trivial things, but when faced with a conflict great enough, the very fibre of a persons being is tested, and how they react proves what kind of person they are. This testing only occurs when one is taken far from their comfort zones, and is such often difficult to see. This is not the case in regards to those involved in the witch trials of Salem in 1692.…
“Conflict theory shows how major patterns of inequality in society produce social stability in some circumstances and social change in orders” (Brym, Roberts, Strohschen, lie 2015:18). I would use conflict theory to explain women running for office in this campaign and why men are more than women in terms of election in different parties. Research and theory associated with studying gender issues propelled the sociology of gender from the margins to become a central feature of the discipline ( Why is it that there are more men involved in elections compare to women? Women have to be given the chance to compete in the election, showing sign of equality amongst men and women which is the fundamental principals of human rights and…
“Peace is not absence of conflict, it is the ability to handle conflict by peaceful means,” this quote by Ronald Regan shows that conflict is an inevitable part of society. Conflict is a fundamental part of life and is always present for every individual throughout their everyday life. Encountering conflict means to face or handle conflict and the ways in which we go about trying to resolve it. Different types of conflict can be local, national, religious, political, economic or cultural. There are a number of reasons why conflict occurs; difference in beliefs, morals, religion and values, fear and unmatched expectations. Conflict has a number of different causes, consequences and ways it can be resolved.…
In our lives conflict plays a huge force in our everyday activities, social events, interaction with our friends and family. But what is the real explanation of conflict? A struggle or destructive force. This description of conflict only touches the surface of what conflict really means. People encounter conflicts almost on a daily basis, whether it is self-brought upon conflict, conflict with what religion, age, gender you are. Who your family and friends are and how they contribute to your life and how their decisions impact you and the others around you. Conflict is an unavoidable thing that we will all face and have already faced conflict in one form or another throughout our lives. How we deal with conflict determines whether it destroys us or not. Conflict, as a force can either crush or build people’s strength people depending on how they deal with it and the repercussions they have to go through post-conflict. It is known to bring trauma and devastation but has also has the ability to bring people together and settle the differences between one another.…
American society today is made up of all three of the theories. In my opinion, these theories all thrive off each other as a whole. I feel that the chain is something like this, Symbolic Interactionism, as well as Functionalism, lead into Conflict Theory. However, I feel that Conflict Theory is the largest component American society is made from, here is why!…
My social issue addresses the availability and accessibility of mental healthcare in Brevard County, Florida. Though it cannot be exactly pinpointed when this became an issue it has been known for many years the lack of resources available to those plagued with mental illness. According to the Professional Research Consultants, Inc., mental illness is one of the primary causes of disability (Professional Research Consultants, Inc., 2013). In addition to this the homeless population also suffers from mental illness, a debilitating 40% (Council on Homelessness, Department of Children and Families, 2013). Without money, transportation, or knowledge how are these people…