Biology presentation
Introduction
Good Morning Everyone. My name is and this is , and . We will be presenting to you on how Light affects gene expression with focus on Maize leaves. This is an unrelated but neat photo of gem corn which is produced as a result of selective breeding.
Anyways, most plants require light in order to grow and conduct photosynthesis. Plants that have been previously grown in the dark are incapable of photosynthesis since they lack chlorophyll and many polypeptides needed for light harvesting. But once they have been exposed to light, over time, reorganization of the plastid membranes occur and all the required enzymes and proteins needed for photosynthesis appear.
So undetectable levels of these proteins suddenly appear and become the most abundant in leaves with the presence of light. The increase of these enzymes are due to increased levels of their corresponding mRNA. Here’s a funny picture of a plant getting ripped. Let’s just assume he’s under light.
Enzymes that are associated with growth in maize that are regulated by light are :
Chlorophyll a/b (LHCP), phosphophenyl carboxylase (PEPCase) and 1,5 – biphosphate carboxylase. 1,5 – biphosphate carboxylase is composed of two different subunits LSu and SSu. We will refer to these enzymes in the abbreviated forms in brackets.
What we will discuss is the schedule of expression of mRNA for each of these enzymes and what is the relative influence of light on the expression of each enzyme?
Colton will continue with the methods.
Conclusion
To conclude, the expression of all these enzymes with the exception of chlorophyll (LHCP) are initially light independent with lower levels expressed in darkness. The RuBPCase subunits are first to appear in all light conditions in both protein and mRNA levels. Upon illumination from darkness , all enzyme and mRNA levels are increased and chlorophyll appears.
Good Morning Everyone. My name is and this is , and . We will be presenting to you on how Light affects gene expression with focus on Maize leaves. This is an unrelated but neat photo of gem corn which is produced as a result of selective breeding.
Anyways, most plants require light in order to grow and conduct photosynthesis. Plants that have been previously grown in the dark are incapable of photosynthesis since they lack chlorophyll and many polypeptides needed for light harvesting. But once they have been exposed to light, over time, reorganization of the plastid membranes occur and all the required enzymes and proteins needed for photosynthesis appear.
So undetectable levels of these proteins suddenly appear and become the most abundant in leaves with the presence of light. The increase of these enzymes are due to increased levels of their corresponding mRNA. Here’s a funny picture of a plant getting ripped. Let’s just assume he’s under light.
Enzymes that are associated with growth in maize that are regulated by light are :
Chlorophyll a/b (LHCP), phosphophenyl carboxylase (PEPCase) and 1,5 – biphosphate carboxylase. 1,5 – biphosphate carboxylase is composed of two different subunits LSu and SSu. We will refer to these enzymes in the abbreviated forms in brackets.
What we will discuss is the schedule of expression of mRNA for each of these enzymes and what is the relative influence of light on the expression of each enzyme?
Colton will continue with the methods.
Conclusion
To conclude, the expression of all these enzymes with the exception of chlorophyll (LHCP) are initially light independent with lower levels expressed in darkness. The RuBPCase subunits are first to appear in all light conditions in both protein and mRNA levels. Upon illumination from darkness , all enzyme and mRNA levels are increased and chlorophyll appears.