D melanogaster Fruit fly Report
BIOL 323-02
TTH 12pm-2pm
Drosophila melanogaster:
Monohybrid Cross & Sex-Linked Inheritance
October 6, 2012
Abstract This lab involved a monohybrid reciprocal cross and a sex-linked cross utilizing Drosophila melanogaster fruit flies. After sexing the flies, placing them in media, and letting them cross breed, we analyzed and recorded the phenotypes that were observed. The P1 flies were anesthetized then sexed by observing their phenotypes and equally distributed into the media we made for their copulation. When a sufficient amount of progeny was present in the culture bottle the P1 generation was killed. After the progeny developed in the F1generation of the sex-linked cross the phenotypes were observed and recorded. After the F1 generation of the monohybrid cross developed the process was repeated to create an F2 generation and the results were observed and recorded. Class data was also included in the results for comparisons. We found that though some of the results for each of the crosses were close to the null hypothesis, they did not accept the probability of the Chi-Square analysis. This means that even though there are expectations for the results, what happens in nature can almost never be controlled completely.
Background
The first experiments utilizing the fruit fly Drosophila melanogaster were performed by Thomas Morgan, when in 1910 he spotted a white-eyed fruit fly in his fly room. However the first reported “mutants” found among the fruit fly were observed in 1907 by Frank Lutz of the Carnegie Institution. He reported different wing patterns and, by 1908, had noticed the proliferation of dwarf mutants. By the 1980s these discoveries have spawned into its own field of study by geneticists sometimes referred to as “Drosophilists”. (Taubes 2012) The purpose of our D. melanogaster Monohybrid Cross experiment was to study the genetic traits based on Mendelian inheritance. In this
TTH 12pm-2pm
Drosophila melanogaster:
Monohybrid Cross & Sex-Linked Inheritance
October 6, 2012
Abstract This lab involved a monohybrid reciprocal cross and a sex-linked cross utilizing Drosophila melanogaster fruit flies. After sexing the flies, placing them in media, and letting them cross breed, we analyzed and recorded the phenotypes that were observed. The P1 flies were anesthetized then sexed by observing their phenotypes and equally distributed into the media we made for their copulation. When a sufficient amount of progeny was present in the culture bottle the P1 generation was killed. After the progeny developed in the F1generation of the sex-linked cross the phenotypes were observed and recorded. After the F1 generation of the monohybrid cross developed the process was repeated to create an F2 generation and the results were observed and recorded. Class data was also included in the results for comparisons. We found that though some of the results for each of the crosses were close to the null hypothesis, they did not accept the probability of the Chi-Square analysis. This means that even though there are expectations for the results, what happens in nature can almost never be controlled completely.
Background
The first experiments utilizing the fruit fly Drosophila melanogaster were performed by Thomas Morgan, when in 1910 he spotted a white-eyed fruit fly in his fly room. However the first reported “mutants” found among the fruit fly were observed in 1907 by Frank Lutz of the Carnegie Institution. He reported different wing patterns and, by 1908, had noticed the proliferation of dwarf mutants. By the 1980s these discoveries have spawned into its own field of study by geneticists sometimes referred to as “Drosophilists”. (Taubes 2012) The purpose of our D. melanogaster Monohybrid Cross experiment was to study the genetic traits based on Mendelian inheritance. In this