Enzyme Activity Ia Essay Example
Year 11 IB Biology IA Report
2011
5/31/2011
Enzyme Activity Experiment
Introduction 3
AIM 3
Materials 3
Method 4
Result 5 Table 1: 5 Table 2: 5 Graph 1 6
Discussion 6
Conclusion 8
Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9
Introduction
Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will go colourless. Here, an alkaline solution of milk, lipase and phenolphthalein will change from pink to colourless as the fat in milk is broken down to form fatty acids (and glycerol) thus reducing the pH to below 8.3. The time taken for this reaction to occur is affected by temperature.
AIM
To investigate the effects of increasing temperature on the activity of enzyme Lipase.
Materials
* Milk, full-fat or semi-skimmed, 5 cm3 per student per temperature assessed * Phenolphthalein in a dropper bottle (see note 1) * 5% lipase solution, 1 cm3 per student per temperature assessed * Sodium carbonate solution, 0.05 mol dm-3, 7 cm3 per student per temperature assessed * Electric hot water baths set to a range of temperatures, each containing a thermometer, a test-tube rack and a beaker of lipase solution. * Ice * Marker pen * Test tube rack * Measuring cylinder (or syringe), 10 cm3, 2 * Beaker, 100 cm3, 2 (for milk and sodium carbonate solution) * Beaker, 250 cm3, 2 (to act as water baths for temperatures below room temperature) * Thermometer * Test tube * Glass rod * Syringe, 2 cm3 * Stop clock/stopwatch
Method
1. Label a test tube with the temperature to be investigated. 2. Add 5 drops of phenolphthalein to the test tube. 3. Measure out 5 cm3 of milk using a measuring cylinder (or syringe) and add this to the test tube. 4. Measure out 7 cm3 of sodium carbonate solution using another
2011
5/31/2011
Enzyme Activity Experiment
Introduction 3
AIM 3
Materials 3
Method 4
Result 5 Table 1: 5 Table 2: 5 Graph 1 6
Discussion 6
Conclusion 8
Bibliography 9 Appendix 1 (Test tube 3- 45 degrees) 9 Appendix 2 (Test tube 1- 5 degrees) 9 Appendix 3 (Test tube 2- 20 degrees) 9
Introduction
Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8.3 phenolphthalein will go colourless. Here, an alkaline solution of milk, lipase and phenolphthalein will change from pink to colourless as the fat in milk is broken down to form fatty acids (and glycerol) thus reducing the pH to below 8.3. The time taken for this reaction to occur is affected by temperature.
AIM
To investigate the effects of increasing temperature on the activity of enzyme Lipase.
Materials
* Milk, full-fat or semi-skimmed, 5 cm3 per student per temperature assessed * Phenolphthalein in a dropper bottle (see note 1) * 5% lipase solution, 1 cm3 per student per temperature assessed * Sodium carbonate solution, 0.05 mol dm-3, 7 cm3 per student per temperature assessed * Electric hot water baths set to a range of temperatures, each containing a thermometer, a test-tube rack and a beaker of lipase solution. * Ice * Marker pen * Test tube rack * Measuring cylinder (or syringe), 10 cm3, 2 * Beaker, 100 cm3, 2 (for milk and sodium carbonate solution) * Beaker, 250 cm3, 2 (to act as water baths for temperatures below room temperature) * Thermometer * Test tube * Glass rod * Syringe, 2 cm3 * Stop clock/stopwatch
Method
1. Label a test tube with the temperature to be investigated. 2. Add 5 drops of phenolphthalein to the test tube. 3. Measure out 5 cm3 of milk using a measuring cylinder (or syringe) and add this to the test tube. 4. Measure out 7 cm3 of sodium carbonate solution using another