Ion Exchange Chromatography
Ion Exchange Chromatography Week 10 TAG
Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column, load your sample and collect the hydronium ion released.
1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the column is exactly level with the top the resin; then close the stopcock.
2. Obtain 10 mL of 3 M HCL regeneration solution using a graduated cylinder and care- fully add the HCL to the column (make sure there is little disturbance to the resin).
3. Open the stopcock and allow the solution level to again drop to the top of the resin.
4. Carefully layer water from squeeze bottle on top of the resin and fill the column to the top.
5. Open the stopcock and allow solution to pass through rapidly into the waste beaker.
6. Add more water as the water level gets closer to the top of the resin and continue until 100 mL of water has been added to the column.
7. Deposit sample into the column and and open the screw clamp to allow the sample to seep into the column until the solution level is even with the top of the resin.
8. Layer water on top of the resin with minimal disturance to your sample solution (about 30 mL of water is needed to wash the sample through the column).
9. Collect the sample in an Erlenmeyer flask. a. Replace waste beaker with a clean Erlenmeyer flask.
b. Open screw clamp and collect the effluent in the Erlenmeyer flask.
c. Control the flow rate to prevent the sample from becoming too spread out on the column, and adjust the flow so that the effluent is dripping out at the rate of one or two drops per second.
d. As the solution level in the column decreases, add more distilled water.
e. After 30 mL has been added, check the pH of the
Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column, load your sample and collect the hydronium ion released.
1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the column is exactly level with the top the resin; then close the stopcock.
2. Obtain 10 mL of 3 M HCL regeneration solution using a graduated cylinder and care- fully add the HCL to the column (make sure there is little disturbance to the resin).
3. Open the stopcock and allow the solution level to again drop to the top of the resin.
4. Carefully layer water from squeeze bottle on top of the resin and fill the column to the top.
5. Open the stopcock and allow solution to pass through rapidly into the waste beaker.
6. Add more water as the water level gets closer to the top of the resin and continue until 100 mL of water has been added to the column.
7. Deposit sample into the column and and open the screw clamp to allow the sample to seep into the column until the solution level is even with the top of the resin.
8. Layer water on top of the resin with minimal disturance to your sample solution (about 30 mL of water is needed to wash the sample through the column).
9. Collect the sample in an Erlenmeyer flask. a. Replace waste beaker with a clean Erlenmeyer flask.
b. Open screw clamp and collect the effluent in the Erlenmeyer flask.
c. Control the flow rate to prevent the sample from becoming too spread out on the column, and adjust the flow so that the effluent is dripping out at the rate of one or two drops per second.
d. As the solution level in the column decreases, add more distilled water.
e. After 30 mL has been added, check the pH of the