Introductory Biochemical Laboratory Worksheet 10 - DNA Melting Curve Course Code: BICH172 Course Name: Introductory Biochemical Laboratory Student Name: CHUNG YUK MAN EMILY Student ID: 10198465 Group Number: 10 Date of Experiment: 16th November 2010 Q1. State the experimental aim and describe the term “hyperchromic effect”. (2 mark) The experimental aims are to study the basic structural characteristic of DNA molecules through UV spectrum and thermal denaturation
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1. Justify the validity of your model include any limitations of your model. My model is valid because I used reliable sources of information‚ as referenced in my bibliography‚ to find information on what DNA replication looks like. The sources showed me what a strand of DNA looks like as well as giving me an explanation of what occurs during DNA replication. As my model is colour coded it makes it clearer as to what is occurring during each step in DNA replication as well as what each element
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DNA replication: DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance. The process starts when one double-stranded DNA molecule produces two identical copies of the molecule. The cell cycle (mitosis) also pertains to the DNA replication/reproduction process. The cell cycle includes interphase‚ prophase‚ metaphase‚ anaphase‚ and telophase. Each strand of the original double-stranded DNA molecule serves as template
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DNA DIGESTION AND ELECTROPHORESIS In this experiment we will be doing a process called as DNA digestion or also known as restriction digest. A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation‚ scientists Hartl and Jones describe it this way: This enzymatic technique can be used for cleaving DNA molecules at specific sites‚ ensuring that all DNA fragments that contain a particular sequence have the
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BIOTECHNOLOGY RECOMBINANT DNA technology 1. This is a modern biotechnological advance ‚ in which a desired gene fragment can be inserted in to a cloning vector and the resulting DNA (Recombinant DNA) can be amplified in suitable host. 2. A vector can be a plasmid‚ cosmid‚bacterophage‚retroviruses‚ animal and plant viruses or artificial chromosomes like YAC‚ BAC‚or HAC.(Yeast artificial chromosome‚ bacterial........) 3. The rec. DNA produced can be amplified or cloned in a suitable vector
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The Importance of DNA Evidence What is DNA? Is it these winding strands that look like ladders or is it what gives a person blonde hair and blue eyes? Actually‚ DNA is both of these things. DNA is a person’s genetic makeup–their hereditary blueprint passed on by their parents. It is a part of almost every cell in the human body. In each cell‚ a person’s DNA is the same; it stays the same throughout their lifetime. DNA is found in skin tissue‚ sweat‚ bone‚ the root and shaft of hair‚ earwax‚ mucus
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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forefront is DNA vaccination. DNA vaccines are bacterial plasmids that encode the polypeptide sequence
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2.5.1 Cell Structure Vocabulary: cell wall‚ centriole‚ chloroplast‚ cytoplasm‚ endoplasmic reticulum‚ Golgi apparatus‚ lysosome‚ mitochondria‚ nuclear envelope‚ nucleolus‚ nucleus‚ organelle‚ plasma membrane‚ plastid‚ ribosome‚ vacuole‚ vesicle Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. What are some of the structures inside a cell that help it to live and perform its role in an organism? ______Mitochondria‚ nucleus‚ ribosome’s. __________________________________________________________
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Since its discovery in the 1980’s‚ the application of DNA has been utilized in many ways. When Deoxyribonucleic Acid‚ or DNA is examined‚ a precise decoding of genetic information is revealed. With the exception of identical twins‚ every person’s genetic code (commonly referred to as a Genetic Fingerprint) is inherited and unique. From Maury advising men everyday on TV “You ARE the Father!” or “You are NOT the Father!” to learning the descendents of former slaves are related to President Thomas Jefferson
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