DNA SPOOLING ISOLATION OF DNA FROM ONION INTRODUCTION This project will demonstrate several basic steps required for isolation of chromosomal DNA from cells. To extract the chromosomal DNA‚ both the cell membrane and the nuclear membrane must be broken open. This is accomplished by disrupting the membranes with a solution of detergent and salt‚creating a cell homogenate. Once the DNA is released from the nucleus‚ it must be protected from nucleases‚ enzymes which will degrade the DNA. Keeping
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EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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DNA fingerprinting is a method that compares the fragments of DNA. DNA fingerprinting was first invented to detect the presence of genetic diseases. Today‚ DNA fingerprinting is used in different ways. DNA is analyzed using a Southern Blot‚ which allows scientists to observe the base pair patterns. DNA fingerprinting can be used in a few different ways. First‚ to find out if the child belongs to a person DNA fingerprinting may be used. When a child is born‚ it inherits the VNTR’s from the father
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1. A saturated fat has one or more double bonds between the carbons in its fatty acid tail: A) True B) False Points Earned: 2.0/2.0 Correct Answer(s): False 2. This organelle keeps DNA from getting tangled in cytoplasmic machinery and isolates it from damaging reactions A) endoplasmic reticulum B) nucleus C) nucleolus D) golgi body Points Earned: 2.0/2.0 Correct Answer(s): B 3. A buffer protects cells against: A) changes in pH B) excessive weight gain C) salt D) heat
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1. Which job role works in a lab environment and might not interact with users of the PCs being repaired? A) Help-desk technician B) Bench technician C) PC service technician D) PC support technician 2. Which job role works on site‚ closely interacting with users‚ and is responsible for ongoing PC maintenance? A) PC support technician B) PC service technician C) Bench technician D) Help-desk technician 3. Which of the following is true about the A+ 2012 certification and exams? A) after
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The amazing advances in health science‚ DNA is transforming the way in which criminal investigations and trials are carried out. DNA evidence and its importance can rest on a single fact: Every individual’s DNA is unique. A person’s DNA profile can be used similar to a fingerprint to link suspects to crime scenes and its victims. DNA profiling—which is also called DNA fingerprinting or even DNA typing—has been responsible for overturning verdicts and saving innocent people from execution. The process
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reported in 2009. The 3 strains of c. Trachamotis are trachoma which affects the eyes‚ urethritis which affects the urethra and cervix and lymphogranuloma venereum‚ which affects the human lymph system. It causes numerous diseases including prostatitis‚ epiditimytisis‚ proctitis and infertility in males and cervicitis‚ pelvic inflammatory disease‚ chronic pelvic pain‚ ectopic pregnancies and infertility in women. It can also cause eye infections in men and women which can lead to blindness. It can
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Recombinant DNA Technology Recombinant DNA technology refers to the ability to isolate specific DNA sequences and alter or manipulate them to produce desired effects. More often‚ recombinant DNA technology is referred to as biotechnology. Recombinant DNA technology is fascinating in that it has developed into a multi-billion dollar industry‚ and completely revolutionized agriculture and pharmaceutical industries‚ all within the past 50 years. According to one account‚ biotechnology was born
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For lazy student like me (Mey pheap) Chapter 1: Discussion Questions 1. What forces have led to the boom in entrepreneurship in the United States? Answer - Corporate downsizing flooding the market with corporate cast-offs‚ an attitude that small is beautiful‚ international economic development due to the fall of communism and the capitalization of former state-owned industries‚ a dream of freedom and independence‚ perseverance in achieving results‚ and the opportunities presented by an ever-changing
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Short answer questions – generally complex by their nature and require knowledge and integration of several different concepts 1. Explain what are the end goals of reproductive cloning and therapeutic cloning. Outline the steps both procedures share and the step(s) specific for each procedure. • Reproductive Cloning- Reproductive cloning is a type of cloning which is performed for the purpose of creating a duplicate copy of another organism. It is accomplished using a process called somatic cell
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