resistant bacterial strains were isolated from contaminated groundwater samples collected from Chakdah and Barasat districts of West Bengal and Jorhat district of Assam. All the isolated bacteria were identified by 16S rRNA gene sequencing. The phylogenetic analysis of the strains using 16S rDNA sequence revealed strains to be under the genus Acidovorax‚ Acinetobacter‚ Rhizobium‚ Enterobacter and Rhodococcus. Isolated bacteria were tested for the presence of plasmids using the modified alkaline lysis
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evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 α hydroxyprogesterone is the metabolite identified having 60 percent of yield. and Bacillus circulans Tex 01-S3c has been found most efficient strain. Key words: - Hydroxylation of Progesterone‚ oil contaminated‚ Bacillus circulans
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procedure is to take place over a time frame of ten weeks conducted by a student team of four. The proposed methodology to take place is to cultivate and purify isolates from the soil‚ find antibiotic isolates‚ isolate the genomic DNA‚ to amplify the 16S rRNA gene segments by polymerase chain reaction‚ and to build a phylogenetic tree. Lantana is a genus of about 150 species of flowering plants popularly used as antirheumatic‚ stimulant‚ antibacterial‚ biologic control and as ornamental plant (Dua
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PCR-based cloning approach. Denaturing gradient gel electrophoresis (DGGE) analysis of PCRamplified 16S rRNA gene fragments from bacteria was used to evaluate the changes in the bacterial community in the process of chalcopyrite bioleaching in a shaken flask system. The results revealed that the bacterial community was disturbed after the addition of chalcopyrite. Phylogenetic analyses of 16S rRNA gene fragments revealed that the retrieved sequences clustered together with the genera Acidithiobacillus
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specialized. Denaturing gradient gel electrophoresis (DGGE) profiling suggested differences in their population compositions and both changed over time. However‚ both showed low level biodiversity‚ and contained several novel bacterial populations. 16S rRNA clone library data and FISH analyses showed that Thermus thermophilus dominated both communities and that of a third ATAD plant in NSW (more than 90% of the total bacterial biovolume in repeated samples taken from each of the three ATAD plants)
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522-2998718; Fax: (+91) 522-2441888 E-mail: bharagavarnbbau11@gmail.com‚ ramnaresh_dem@bbau.ac.in Abstract In this study‚ Crystal violet dye decolorizing bacterial strain SJ4 was isolated from textile wastewater and characterized as Aeromonas hydrophila by 16S rRNA gene
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The adaptive part is comprised of the repair process and the maintenance of the cell. The aim of the adaptive part is to sustain the cell from the damages caused by the temperature shifts and enable it to continue to grow. Repair process is crucial‚ especially when the DNA was destructed by the adverse impact of the rapid change in temperature. For example‚ when the cell was exposed to 0oC‚ the gene encodes for PP5 and ATM were regulated. The function of the PP5 and ATM is the DNA damage control
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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for clinical chemistry evaluations was placed in tubes devoid of anticoagulant‚ allowed to clot at room temperature‚ and centrifuged‚ and serum was separated. Blood collected from all 10-mice/exposure groups was used for determination of each of the 16 haematology parameters. Adequate plasma samples obtained from another 10-mice/exposure group were available for determination of each of the 11 clinical chemistry parameters. Necropsy was performed on all treated and control animals. Selected organ
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Opin. Microbiol. 2‚ 265–269. Margesin‚ R.‚ Schinner‚ F.‚ 2001. Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5‚ 73–83. Matheson‚ A.T.‚ Louie‚ K.A.‚ Tak‚ B.D.‚ Zuker‚ M.‚ 1987. The primary structure of the ribosomal A-protein (L12) from the halophilic eubacterium Haloanaerobium praevalens. Biochemie 69‚ 1013–1020. Mavromatis‚ K.‚ Ivanova‚ N.‚ Anderson‚ I.‚ Lykidis‚ A.‚ Hooper‚ S.D.‚ Sun‚ H.‚ Kunin‚ V.‚ Hugenholtz‚ P.‚ Patel‚ B.‚ Kyrpides‚ N.C.‚ 2009. Genome
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