affect the rate of diffusion. __The size and the concentration. ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? __ The molecules were to large to go through. My results matched._ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? __The albumin was not able to go through and the glucose did. I predicted
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Activity FOUR There is a direct relationship between the MWCO and the filtration rate; the higher the MWCO‚ the higher the filtration rate. Powdered charcoal is the solute that did not appear in the filtrate when using any MWCO membranes. As pressure increases‚ the filtration rate also increases‚ as long as the MWCO does not decrease. Since we have no control over the MWCO of a living membrane‚ we can increase the rate of filtration by increasing the pressure. The molecular weight
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of the molecule diffusing through the membrane 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? * Because the pores of the membrane were not large enough * I predicted this correctly. 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? I predicted that only glucose would diffuse through
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Natalie Salaverria 01/21/11 LAB EXERCISE I After the first exercise : Simulating Simple diffusion Which Materials diffused from the left beaker to the right beaker? NaCl ‚ Urea ‚ Glucose Which did not ? Albumin Why ? Albumin’s Composition or charge was too great to diffuse through the membrane. After the 2nd activity : Simulating Dialysis What happens to the urea concentration in the left beaker (the Patient)? It diffused to the right beaker Why does this occur? The excess
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was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? a. Urea was unable to diffuse through the 20MWCO membrane because urea is a big molecule and the 20MWCO membrane pore size was too small for Urea. My predication was accurate‚ as I predicted that Urea would not be able to diffuse through the 20MWCO membrane 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results
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increase in blood pressure Experiment Data: Run Number 1 1 1 1 2 2 2 2 3 3 3 3 4 4 4 4 Solute Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal Na+ ClUrea Glucose Powdered Charcoal MWCO 20 20 20 20 50 50 50 50 200 200 200 200 200 200 200 200 Pressure 50 50 50 50 50 50 50 50 50 50 50 50 100 100 100 100 Filter Rate 1.00 1.00 1.00 1.00 2.50 2.50 2.50 2.50 10.00 10.00 10.00 10.00 20.00 20.00 20.00 20.00 Residue present present present present
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FACILITATED IS MOVEMENT WITH THE HELP OF CARRIER PROTEINS 3. What is the main difference between facilitated diffusion and active transport? ACTIVE TRANSPORT USES ATP 4. In the “Simple Diffusion” experiment‚ which solute(s) passed through the MWCO 20 membrane? NONE Why? SOLUTES MASSES ARE TO BIG 5. List three examples of passive transport mechanisms. DIFFUSION FILTRATION OSMOSIS 6. Describe the relationship of solute concentration to solvent concentration in osmosis. CONCENTRATION
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During which phase of the cell cycle does DNA duplication‚ or replication‚ take place? – interphase When a double helix of DNA is replicated‚ two complete helices are formed. Together‚ these helices are called sister __________. – chromatids During which phase of mitosis do sister chromatids line up at the center of the cell? – metaphase During which phase of mitosis do the sister chromatids move apart? – Anaphase During which phase of mitosis do nuclear envelopes and the nucleoli reappear?
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imple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. Facilitated
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change in osmotic pressure across a membrane? The solutes can diffuse through the pores and the concentration of solutes is the same on both sides of the membrane. With the experimental conditions set at 10 mM glucose and 9 mM albumin‚ and the 200 MWCO membrane in place‚ which of the following is true? The net movement of water is toward the albumin. Which of the following can be true of both active transport and facilitated diffusion? Lipid-insoluble solutes are transported across the membrane
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