Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed for transfection
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Photosynthesis Lab Report Purpose: To research the effect of different wavelengths (colors) of light on plant growth during photosynthesis. Background Information: Photosynthesis is the process by which sunlight is captured by chloroplasts within plant cells and turned into energy. This energy is used to help the plant grow roots‚ leaves‚ flowers and fruit. Energy is required by living things
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Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
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LAB FIVE CELL RESPIRATION INTRODUCTION Aerobic cellular respiration is the release of energy from organic compound from organic compounds by metabolic chemical oxidation in the mitochondria within each cell. Cellular respiration involves a series of enzyme-mediated reactions. The equation below shows the complete oxidation of glucose. Oxygen is required for this energy-releasing process
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CHM130 Lab 3 Identification of Chemical Change Name: Paige Miller A. Data Table (24 points) Place your completed data table here: Reactants Predictions Before Combining Observations After Combining Starch + I2 I2 will turn Starch black I2 turned starch black Food coloring + NaOCl NaOCI will turn blue Coloring and NaOCI did not combine‚ NaOCI separated to the bottom Food coloring + CH3COOH CH3COOH will turn blue CH3COOH darkened the blue color Food coloring + NaOCl + CH3COOH Will
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Biophysical Ecology Abstract: There are many patterns that can be observed throughout our environment. In this experiment‚ the temperatures of organisms in a biophysical environment were analyzed to see if a pattern could be recognized that was related to the behavior of an ectotherm. An ectotherm is an organism that relies on the environment to regulate its body temperature. Organisms such as Pseudemys (turtles) and Lacertilia (lizards) are examples or ectotherms. After arriving at Maxcy Gregg
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The objective of this macromolecules lab was to identify the presence any of the major macromolecules in various every day food items. The three macromolecules that this lab was carried out for were carbohydrates‚ lipids‚ and proteins. There were five different experiments conducted and each of those experiments had one factor in common‚ they all had the same controls. The controls in this lab activity were already set for the lab activity. The controls were the distilled water and the baking soda
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Title- Protista Lab Concept- the concept of this lab was to explore what is in the pond water as living organisms and try to identify what it is. Some types of Protista are multi-cellular like giant kelp. Although they look much like plants‚ multi-cellular protists lack specialized tissues. Being eukaryotes‚ they have a membrane-bound true nucleus with linear chromosomes‚ and they have membrane-bound organelles. The kingdom Protista contains all eukaryotes that are not plants‚ animals‚ or fungi
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Transpiration Formal Lab Report By Jessica Tran Introduction When water is transported from the roots to the mesophyll cells in the leaves‚ it is evaporates out the stomates‚ called transpiration‚ to create a lower osmotic potential. Osmotic potential is the part of the water potential of a tissue that results from the presence of solute particles. Even though the stomates open to release water‚ it also brings in carbon dioxide to produce sugar and oxygen through a process of photosynthesis
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absorbance‚ which was used to calculate initial cell density. The caps on each of the samples were then loosened to allow movement of gas in and out of the tube. After a week of growth under fluorescent lighting‚ the tubes were inverted for mixing and measured for absorbance again. The values were used to calculated the corresponding final cell density. The calculations of initial and final density of cells were used to determine the algal growth rate of cells per milliliter per
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