Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
Free Enzyme Amino acid Functional group
polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell and is named according to the substrate they specifically affect. An enzyme works best under optimal conditions pertaining to temperature‚ pH level
Premium Protein Metabolism DNA
affecting Enzyme Activity: The effect of pH on enzyme activity Background Knowledge: An enzyme is a biological catalyst – which speeds up the reaction rate‚ without itself getting altered. Enzymes are proteins with long polypeptide chains that are folded up into three – dimensional shapes. An enzyme acts on a substrate to convert the substrate into a product useful for the organism.The active site is a special region on the surface of the enzyme where the substrate binds to the enzyme.
Premium Enzyme Chemical reaction Catalysis
My Thesis Statement: This essay argues that humans have used globalization as a tool to reach their ends and in the process animals are a very easier means at their disposal to realise their ends‚ thus leaving animals bereft of all their life rights and entitlements. How globalization have encouraged humans to disregard the moral rights of animals? Animals are used in food as much as they are used in sports in the Middle East‚ in that when camels are used for racing using robot jockeys who whips
Premium Animal Farm The Animals George Orwell
"Could You Live with Less" Stephanie Mills in her essay "Could You Live with Less" states her opinion about technology. According to this author‚ "technology comes at a serious cost to the planet and most of its people" (Mills 2). It is hard to argue with this author about how we can restrict our needs by using less technology. However Mills believes that technology has a more negative then positive influence in our lives. Mills is proud to live simply‚ restricting herself to technology. This
Premium Human Science Mobile phone
Thomas’ motivational speech How Bad Do You Want It? There was a young man‚ you know‚ who wanted to make a lot of money and so he went to this guru‚ right. And he told the guru you know I wanna be on the same level you are‚ and the guru said if you wanna be on the same level I’m on‚ I’ll met you tomorrow at the beach. So the young man got there 4 A.M. he’s all ready to rock n’ roll. Got on a suit‚ should of wore shorts. The old man grabs his hand and said: How bad do you wanna be successful?
Premium
Temperature‚ and Enzyme Concentration on Catalase Reaction Rate II. Introduction Enzymes are used to increase the rate of specific reactions in the body. Catalase‚ a specific enzyme‚ speeds the breakdown of hydrogen peroxide‚ a toxic chemical produced by cells in the body‚ into water and oxygen (Cain and others‚ 2010). The oxygen can be observed as bubbles coming from the reaction site. Catalase is found in many living tissues of organisms‚ including chicken liver. The purpose of this experiment is to determine what changes in pH
Premium
reminds you of? How would you define freedom? O) Everything can be defined with its opponent‚ therefore captivity comes to my mind when I think of freedom. Being behind walls is not the only thing that captivity reminds me of. Actually everything that limits us in our daily lives‚ everything that restricts our lives can be considered as something that captures us. Therefore a world without those can be considered as a free world‚ though it cannot be fully possible. B) Then how would you define freedom
Premium Free will Political philosophy Human rights
Nicholas Destino Professor Thomas English 101 10 November 1997 Do Animals Have Emotions? Somewhere in the savannas of Africa a mother elephant is dying in the company of many other pachyderms. Some of them are part of her family; some are fellow members of her herd. The dying elephant tips from side to side and seems to be balancing on a thin thread in order to sustain her life. Many of the other elephants surround her as she struggles to regain her balance. They also try to help by feeding
Premium Chimpanzee Ethology Psychology
CGB 514 (CYTOGENETICS) TOPIC: PLANT CELL ORGANELLES AND THEIR FUNCTIONS‚ THE DIFFERENCES BETWEEN THE PLANT AND ANIMAL CELL BY ONUMAEGBU BRIGHT C MOUAU/08/11351 LECTURE: DR J N EKA February 2013 Dedication I dedicate this work and the articles therein to
Premium Cell Organelle Eukaryote