Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Less Personal Interactions Electronic and computer interactions are less time consuming‚ do not take as much thought and effort‚ and is less involving. When electronics increased in our generation‚ face-to-face interactions decreased. Electronic interactions has made people lazy‚ and caused a decrease in personal encounters. Although electronics have made a few positive impacts in one’s daily life‚ electronics have caused more damage and negative impacts than positive. Social media is one of
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of knowledge‚ however there have been some experiments carried out that have had serious negative consequences on their test subjects. Two experiments of this type are the ‘Little Albert’ experiment where a child was conditioned to fear a white rat to test whether fear is innate or conditional. The second experiment was carried out on young orphans to find out if stuttering was a genetic trait or something that can be cured or induced. Both experiments left their subjects permanently affected and
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Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 3. To determine the effect of ascorbic acid on Peroxidase. 4. To determine the level of specificity of Peroxidase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 5. To calculate the effect of the substrate acetaldehyde concentration
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Josef Mengele was chief doctor at the Auschwitz concentration camp. Dr. Mengele’s experiments demonstrated his disregard for human life and the basic rights of the jews by using them as tools for research. Josef Mengele known as‚ “The Angel of Death”‚ treated his patients as animals and used them to improve health practices on the German soldiers‚ and in some cases just out of curiousity as to what would happen if he did something to someone. Mengele’s experiments lead to the deaths of over 400‚000
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Enzymes control the rate of metabolic reactions‚ they act as biological catalysts‚ which means they are used but not used up and they also control the speed of the reaction. Enzymes are proteins which means that anything that disrupts this structure such as high temperature or change in pH will affect the enzyme activity. There are many factors affecting enzyme action for example temperature effects them‚ if the Increasing the heat gives molecules more kinetic energy so they vibrate this can then
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Patrick McCrystal Enzymes: Natural Catalysts Enzymes are catalytic proteins‚ meaning they speed up chemical reactions without beingused up or altered permanently in the process. Although various enzymes use different methods‚all accomplish catalysis by lowering the activation energy for the reaction‚ thus allowing it tooccur more easily. Enzymes have very specific shapes (conformations). Part of the conformationis the active site of the enzyme‚ where the actual catalysis occurs. The specific molecule
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Melanie McGivern. Access to nursing Group 2 Effects of pH on enzyme activity Contents Front cover Aim Introduction Hypothesis Prediction Variables Materials Methods Results Discussion Conclusion Bibliography Aim The aim of the experiment is to see the enzyme amylase catalyse starch in a chemical reaction. | | Introduction Enzymes are proteins. They act as catalysts‚ allowing chemical reactions to take
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and citrate. There are different types of cheeses because there are many enzymes that are used to make the cheese‚ and also there are endless forms of molds that help age cheese giving each cheese its distinctive taste‚ texture and appearance. Enzymes are used in many food production processes to speed up the chemical reactions‚ which reduces water usage and energy consumption. The original objective of cheese production was to conserve the principal constituents of milk the lipids and caseins‚ although
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