Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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Can you be the master of your own destiny? It is said in the Gayan‚ ’The present is the reflection of the past‚ and the future is the re-echo of the present.’ Destiny is not what is already made. Destiny is what we are making. Very often fatalists think that we are in the hands of destiny‚ driven in whatever direction in life destiny wills; but in point of fact we are the masters of our destiny‚ especially from the moment we begin to realize this fact. Among Hindus there is a well-known saying that
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1. You are looking at a Euglena using the x10 objective lens. You rotate the nosepiece around to x40 but the specimen is not visible. Describe what you should do next? Start by using the fine adjustment to attempt to focus on the Euglena. However‚ if it is still not visible‚ return to the x10 objective lens and use the fine adjustment to ensure the Euglena is as sharply focused as possible. Then‚ use the stage control to re-position the slide so the Euglena is directly in the centre of the lens.
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Many professions contain a duty of care‚ these include health‚ social care and education. Duty of care involves an obligation that a person in a specific role has to ensure that mo harm is made to a child or young person during a particular task. This involves giving the appropriate care and attention‚ watching out for potential hazards‚preventing mistakes or accidents and making the right wise choices about the certain steps taken in a role. If duty of care is not met in a role the the person who
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Mitochondria is a double membrane bound organelle in charge of energy production. Mitochondria have their own DNA and manufacture their own ribosomes. They produce mitochondrial DNA and nuclear DNA. Mitochondria can be considered a dynamic organelle because their core function is ATP production. Mitochondria produce about ninety percent of the body’s energy. They migrate within the cells and continuously divide and fuse with each other (Santos‚ 2010). They are responsible for producing energy to
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Nucleus * Largest organelle * Houses the majority of the cells genetic information. * Has instructions for making proteins - * Some of which regulate cell activity Within the nucleus- Structure - | Function - | * Dark patches shown when stained are called Chromatin(s) | * Chromatins consist of DNA and proteins. In mitosis condense and become visible chromosomes. | * Surrounded by Nuclear envelope | * Phospholipid bi-layer with fluid between them with pores
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What contribution can you make to the learning environment? Every educational institution aims to provide a quality‚ effective and engaging learning experience for all its students. This aim has always been central to every institution’s objective to achieve a significant success. And in this learning experience‚ the learning environment is one of its critical components. Those superior-quality services to support learning depend on effective collaboration between the academic staff responsible
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most obvious organelle in a eukaryotic cell. Virtually all eukaryote cells have a nucleus (red blood cells lose theirs and so are enucleate for most of their life-span of 180 days). It is surrounded by the nuclear envelope‚ a double membrane with contains a large number of pores. Molecules enter and leave the nucleus by these pores. The two lipid bilayers of the double membrane are separated by a gap of 20 to 40 nm‚ called the perinuclear space. The nucleus contains the cell’s DNA. This complex molecule
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Experiment IV: Study of Krebs cycle using Mitochondria from Mung Bean Seedlings INTRODUCTION The mitochondria is a very important organelle in the plant cell because it carries out very important cellular reactions in the cell like the Krebs cycle and oxidative phosphorylation which is how the plants produce ATP from the pyruvate produced through glycolysis (Meyer and Millar‚ 2008). Glycolysis produces a net of 2 ATP for the plant which is not enough for the cell to function while the Krebs cycle
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curricular activities go hand in hand in our endeavour to promote an all round development in a child. In this connection‚ we are pleased to inform you that we are having a ‘Fancy Dress Competition’ for the Kindergarten students. Though it was recorded in the school diary as 29th and 30th of June 2011‚ due to unavoidable reasons‚ we will be holding the competition between the 3rd and 5th of July‚ 2011. In this regard‚ please make a note of the following grades and dates on which it will be conducted: *
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