Aim: To verify the buffering capacity of a phosphate solution using a pH meter and construct a titration curve of an amino acid to determine the pka values of its ionisable groups to identitfy an unknown amino acid. Method: The ratio of [HPO42-] to [H2PO4-] required to produce buffer solutions at pH values 5.9‚ 6.9 and 7.9 were calculated. 0.1M of H2PO4- and 0.1M HPO42- were used to mix appropriate volumes to 25mL of each of the buffer solutions. The calibrated pH meter was used to measure
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Introduction to Biochemistry Biochemistry : a multidisciplinary science that explores the chemistry of living organisms and molecular basis for changes occurring in living cells. Principal areas of Biochemistry 1. Struture and Function of Biomolecules Based on structure depend the function (structure function relationship) Example: Hemoglobin S differs from regular adult hemoglobin (hemoglobin A) by just one single amino acid. A valine replaces a glutamine in the 6th position of the beta
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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Biochemistry of Photosynthesis Overall Word Equation: Water+ Carbon Dioxide( Carbohydrates+ Oxygen ((: light + chlorophyll) 1. Light Dependent Stage/ photochemical reaction/ light reactions: Word Equation: Water ( Hydrogen ions + Oxygen ((: light + chlorophyll) Site: On the Thylakoid membrane of the chloroplast Major events: • Light absorption o Chlorophyll in the thylakoids absorbs light. The
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Analysis 5. After the addition of silver nitrate‚ a dirty white solution with brown layer on top was produced. 6. Yeast‚ especially brewer’s yeast‚ contains high levels of purines. Ribose Analysis 7. After boiling‚ a brown solution was produced. 8. ---------------------------------------------------------------------------------------------------------- 9. The result with 0.1% Glucose was a flesh-colored solution. 10. Ribose is one of the many vital carbohydrates necessary for our bodies to
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the amino acid sequence of the entire polypeptide? A) Trypsin digestion: 1) Gln-Met-Lys 2) Gly-Met-Asp-Ile-Lys 3) Phe-Ala-Met-Lys 4) Tyr-Arg B) Cyanogen bromide treatment: 5) Asp-Ile-Lys-Gln-Met 6) Lys 7) Lys-Phe-Ala-Met 8) Tyr-Arg-Gly-Met 4) While on an expedition to the Amazon jungle‚ you isolate a polypeptide you suspect of being the growth hormone of a newly discovered species of giant spider. Unfortunately‚ your portable sequenator was so roughly handled at the
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References: John R Holum‚ 1996‚ 2nd ed‚ organic and biological chemistry‚ von Hoffman press. Monique Laberge phd‚2008‚ 1st ed‚ essential chemistry‚ Chelsea house publications. Nelson David‚ 2005‚ 1st ed‚ principles of biochemistry‚ WH freeman company. www. Wikipedia.com? Cori /cycle Lowenstein J.M‚ 1969‚ 1st ed‚ citric acid cycle‚ Boston academic publication. Bhagavan et al‚ 2006‚ 1st ed‚ coenzyme Q10‚ intake and absorption‚ free radical research. Zmitek et al‚ 2008‚ improving
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isopropanol precipitation method. Restriction enzymes are enzymes that selectively cut a DNA molecule at a particular place called restriction sites. Restriction enzymes also recognize a specific sequence of nucleotides‚ which vary between 4 and 8 nucleotides‚ in particular‚ palindromic. These restriction enzymes were kept in a 50% glycerol buffer that does not freeze at -20 oC. The four available enzymes used for this experiment were EcoRI‚ AvaI‚ HincII‚ and RsaI. The lengths of the DNA fragments
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True False 5. In the blood hydrophillic hormones are often bound to transport proteins. True False True False 6. People with Type I diabetes mellitus have normal insulin levels. True False 7. Taste cells are not neurons. True False 8. Hypoglycemic hormones are secreted in response to excessively low blood glucose levels. True False 9. Suppose you were
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I. Changes Leading to Exploration A. Expulsion of Moors in Spain and reunification of the country under Catholic rulers Isabella and Ferdinand caused an age of exploration and expansion. B. After German Monk Martin Luther posted his 95 theses against church abuses‚ there was a powerful movement away from the Catholic Church called the Protestant Reformation‚ causing the Roman Catholic countries to secure their power in the world by converting
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