Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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Grant Proposals HSM/270 June 5‚ 2013 Program planning is a process that shows the needs of a community and the reason and purpose for the programs an organization wants to set up to address that need. Through program planning; goals‚ objectives‚ activities‚ and evaluations are set up to provide the framework for the grant proposal to be written. A well written grant proposal will show the reader that the organization writing the proposal truly understands the problems within a specific community
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Running head: TRANSFORMATION OF ROME TO AN EMPIRE Transformation of Rome to an Empire Austin Lamb University of Phoenix Transformation of Rome to an Empire How did Rome convert from a Republic to an Empire? The process consisted of a series of instances that brought Rome to what we know it of today. Rome becoming an Empire was a major historical event which included: the transformation from a Republic to an Empire‚ several events that were significant‚ and many characteristics
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to Writing a Grant Proposal Cheryl A. Boyd HSM/270 March 3‚ 2013 Patricia Zindler Steps to Writing a Grant Proposal Writing and obtaining grants will require more than just a great idea‚ and a source to obtain funding. An organization needs to be well managed‚ and have a clear understanding of its mission and purpose. Funders want to know that the organization they pick to give funds has a clear vision of its mission‚ and knows how to use available resources efficiently. The grant proposal should
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to kill an elephant that had run rampant in lust throughout a village. In its rampage the elephant destroyed a truck‚ a hut‚ and killed a villager. The villagers were obviously upset about the situation and he was called upon to restore the order before anything or anyone was hurt. Throughout the ordeal‚ he decided that it was best to kill the elephant. His reasons for doing so‚ however‚ were not as clear-cut. He said his ultimate decision was to not look bad in front of the villagers that gave him
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LESSON PLAN Class : 6 Azam Date : 21 October 2012 Day : Sunday Time: 9.15 a.m. – 10.15 a.m. No of Pupils : 30 Topic : Short story : Beauty and the Beast Skills : 3.3‚ 3.6‚ 3.9‚ 3.10 and 4.3 Language Aspect : Reading comprehension‚ grammar‚ vocabulary Learning outcome: At the end of the lesson‚ students should be able to : 1. Indicate the sequence of story that took place by rearranging the paragraph accordingly. 2. Summarise the story by filling in the blanks with
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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don’t understand. This pen is magical‚ but not the good kind of magic you see on television. This is the evil magic.” “Evil? Well how can that be? It’s just a pen.” “This pen was given to me by a witch several years ago; she told me that it would grant me three wishes. The way the pen works is that you have to write down the wishes and don’t get me wrong they do come true‚ but you must be very careful about how you wish for them. One night when I was mad‚ I wished to go somewhere
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“THE TRANSFORMATION OF BRITISH LIFE. 1950-2000” This book has been written by Andrew S. Rosen‚ who has been named president of Kaplan‚ Inc.‚ a leading provider of educational services to individuals‚ schools and businesses. The author has chosen his title carefully: British life in the second half of the twentieth century was transformed but not changed. The book talks about some aspects as life‚ transport and other‚ like customs. The religion‚ for example. There was a division of that. On
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“Grant and Lee: A Study in Contrasts‚” written by Bruce Catton compares and contrasts the characteristics and lives of two leaders of the Civil War. Ulysses S. Grant and Robert E. Lee‚ two very strong and very different generals‚ met on April 9‚ 1865 at Appomattox Court House in Virginia to bring to a close the Civil War. By this time‚ America had become a country that was starting over with the simple core belief in equal rights for everyone. Lee‚ who is from Virginia‚ had very traditional and
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