Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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CXC School Based Assessment (Year 2013-2014) Name of Candidate:________________ School: __________________________ Centre #:________________________ Candidate#:______________________ Teacher: ________________________ Acknowledgement I would like to thank God for giving me the skills‚ resources and knowledge to carry out this investigation. I would also like to thank Mrs Johnson‚ my social studies teacher‚ and the social studies department for giving me the relevant information
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different organisms. Use the chart to compare the amino acid sequence in humans to the sequences of the other organisms listed. For each organism‚ identify any amino acid that is different or missing when compared to the amino acids in the human sequence. 1. Click on the following link to open the activity chart: Amino Acid Sequences in Cytochome-C Proteins. 2. Compare the human/chimpanzee to each other organism‚ entering the number of differences in the chart (Tip: the next comparison is human/chimpanzee
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food substance presence in food sample Assumption: Controlled: using the same amount of food sample in different test Procedure: Solid: Grind the food with distilled water. Squeeze the ground materials through layers of muslin. Liquid: Put 1 cm3 of the liquid food sample into test tubes. -grease spot test: A drop of liquid food sample was added to a filter paper and was let to dry for 5 minutes. The filter paper was held up to the light and any translucent spot was observed.
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KOLEJ MATRIKULASI MELAKA 78300 MASJID TANAH‚ MELAKA Guidelines for Biology Experiments 1.0 Attendance 1.1 Attendance to practical sessions is COMPULSORY. 1.2 If you cannot come to the class due to ill health or emergency excuses‚ please inform the lecturer EARLIER‚ so that another practical session can be arranged for you ON THE SAME WEEK. 2.0 Lab coat 2.1 Wearing lab coat is COMPULSORY. 2.2 Put on the lab coat throughout the class. 3.0 Jotter 3.1 Jotters should contain the summary on the PROCEDURES
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also be controlled by closing all doors and windows in the classroom‚ in order to maintain the room temperature and not disrupt the experiment. Apparatus: - Timer - 1 sheet of squared paper (square’s area 1cm2) - 1 non-heated lamp - 1½ of a Petri dish - 1 ruler - 1 plastic spoon - 5 Porcellio scaber Method: 1. Collect the materials listed above. Next‚ plug-in the non-heated lamp and position it so that it is 19cm away from where the Porcellio scaber will be placed (measure this
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the Glucose into CO2 • Each step catalyzed by a specific enzyme. • It is a cycle because the product of step 8 is the reactant in step 1 (oxaloacetate). Substrate Level Phosphorylation 1 14-01-05 Electron Carriers (coenzymes) H Krebs Cycle H • The overall chemical equa%on is: 2 oxaloacetate + 2acetyl-‐coA + 2ADP + 2P + 6NAD+ + 2FAD à
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Exercise 1: Dissolved oxygen is oxygen that is trapped in a fluid‚ such as water. Since virtually every living organism requires oxygen to survive‚ it is a necessary component of water systems such as streams‚ lakes and rivers in order to support aquatic life. The dissolved oxygen is measure in units of ppm—or parts per million. Examine the data in Table 2 showing the amount of dissolved oxygen present and the number of fish observed in the body of water the sample was taken from; finally‚ answer
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The Effects of Osmosis and Diffusion The experimentation of last week’s lab was in order to test the many effects of diffusion and osmosis amongst four experiments. One such experiment was testing the effects of molecular weight on diffusion in relation to the use of Agar. The methods performed included the use of two acids‚ HCl and acetic acid. Both acids were placed into an Agar-filled dish and‚ over increments of 15 minutes‚ data collection was taken based off the diffusion rate and the
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1. By exhaling out carbon dioxide into the straw on top of the bromothymol blue indicator‚ the solution will turn the color blue into a yellowish-green color. When there is a presence of something that is acidic the BTB indicator changes its color into yellow/green. So in this case‚ the water with BTB and carbon dioxide exhaled into the flask creates a carbonic acid. As an aerobic activity increase than the carbon dioxide rate increases as well. 2. By exercising more‚ one’s body requires more
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