Molecular Biology Lab Report Mapping DNA using Restriction Enzymes Ava II and Pvu II to cut Bacterial DNA Abstract The objective of this project is to map bacterial DNA‚ which is derived from E. coli‚ using restriction endonucleases with gel electrophoresis. The DNA fragments‚ after cutting has occurred‚ are separated using agarose gel electrophoresis. The DNA fragments are placed in the gel‚ and an electric current is run through the matrix of the gel-like agarose. Migration of the fragments
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Biology GCSE Coursework: Osmosis in Potato Chips Skill Area P: Planning Aim: To investigate the effect of varying concentration of a certain sugar solution on the amount of osmotic activity between the solution and a potato chip of a given size. Hypothesis: Osmosis is defined as the net movement of water or any other solution’s molecules from a region in which they are highly concentrated to a region in which they are less concentrated. This movement must take place across a partially
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Osmosis Lab: Potatoes and Elodea Background Information: Cells have a need to regulate their internal environment. They need to be able to this because cells often find themselves in environments where the concentration of dissolved solids outside the cell is different from the number of dissolved solids inside the cell. Since the solids can not move across the membrane‚ the cell responds by moving water either into or out of the cell in an attempt to balance the number dissolved particles.
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Osmosis and Water Potential Year 11 Emary Venter INTRODUCTION: The cell is the basic unit of living things‚ and is made up of multiple organelles. Organelles are membrane bound subdivisions‚ each specialised for a specific function. This experiment looks at the Plasma Membrane‚ which is a semipermeable layer surrounding the cell. It’s primary job is to control what goes in and out of the cell. Molecules can move across this membrane in either an active movement or a passive movement
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Physioex 9.0 Review Sheet Exercise 1 Cell Transport Mechanisms and Permeability Name: Jeffery Cook Lab Time/Date: 12pm 2/09/2015 Activity 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. a. Two variables that affect the rate of diffusion are size and whether or not there is an electrical or concentration gradient. 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction
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Biology 1010 ANATOMY STUDY GUIDE Ionic bond: A chemical bond resulting from the attraction of oppositely charged ions. Covalent bond: A strong chemical bond in which to atoms share electrons. Cell components (explain their functions): Classification scheme of living organisms: Enzymes (explain functions) : Diffusion: Osmosis: Active Transport: Steps of cellular respiration: How does the body grow and maintain itself? The body is made up of tiny cells - for example
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Physiology • Anatomy: The study of structures The Human Body: An Orientation • Subdivisions: – Gross or macroscopic anatomy (ex: regional‚ systemic anatomy) – Microscopic (ex: cytology‚ histology) – Developmental (ex: embryology) CH 1 1 Overview of Anatomy and Physiology 2 Overview of Anatomy and Physiology • Essential tools for the study of anatomy: – Mastery of anatomical terminology – Observation – Manipulation – Palpation – Auscultation • Physiology: The study
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had two different purposes. The first is to teach us about freezing points. This lab was designed to show us the freezing point of a pure solvent‚ in comparison to a solvent in a solution with a non-volatile solute. The second goal of the experiment is to teach students about osmosis. In the experiment‚ we got to observe osmosis as well as understand dialysis. Procecure : Part 1. Colligative Properties 1. Create a water bath by filling ½ of the 100 mL beaker with cool water‚ adding crushed
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Biology 1. If an interphase cell is treated with cyanide (a metabolic poison)‚ the cell does not divide by mitosis. However‚ if cyanide is added right after mitosis has started‚ the same cell completes mitosis. Which of the following explains this observation? A. Metabolic activity ceases during mitosis B. Cell division does not require metabolic activity C. Energy required for mitosis is produced and stored in the cell during interphase D. Mitotic cells make factors that make them resistant to
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Biology Lab Report (F.4) * Objective: To study the transpiration of a leafy shoot using the bubble potometer * Materials and apparatus: * Bubble potometer * Leafy shoot * Scissors * Basin with water * A fan * Plumbing tape * Method: Normal Condition: 1. The bubble potometer was filled with water. 2. The stem of a leafy shoot was cut under water. 3. The bottom of the stem was wrapped by plumbing tape. 4. The stem was fitted onto the photometer
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