IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Lab Report: Scientific Method Your Name: Alyssa Melincoff Purpose of this Lab What is the goal of this lab? What question is it trying to answer‚ or what problem is it trying to explain? To find out how table salt affects the boiling temperature of water. Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. What hypothesis can you generate about the effect of adding salt to water? Adding
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Name: Angel Simon Pre–Lab Question Ocular lens 1. Label the following microscope using the components described within the Introduction. Experiment 1: Virtual Magnification Exercise Post-Lab Questions 1. At what magnification do you first notice the ragweed pollen? Answer: 1000 2. Which is bigger‚ a rhinovirus or E. Coli? Answer: E. Coli 3. Based on the magnification‚ how many of the E. Coli can fit into the same space as the
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The purpose of this lab was to observe the rate of osmosis and diffusion‚ as well as the effect of molecular size of the particles on this rate. Part I of the lab was a demonstration of osmosis and diffusion‚ that dealt with raisins in different liquid environments‚ each with a different concentration of sugar. Part IV of the lab was using the same idea as the demonstration‚ by putting objects in different concentrations of a substance; in this case elodea leaves in salt water. In both cases‚ the
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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Lab 10 Name: Complete the following then save with your name and attach to the dropbox for lab 10. Data Tables and Post-Lab Assessment Experiment 1 Post-Lab Questions 1. Label each of the arrows in the following slide image: A. Chromosomes B. Nucleus C. Cytoplasm D. Cell wall 2. What is the difference between the rough and smooth endoplasmic reticulum? 3. Would an animal cell be able to survive without mitochondria? Why or why not? 4. What
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Osmosis and Scientific Method Lab Report Abstract The purpose of the lab is to see how different size potatoes have faster water movement depending on the concentration of sucrose it was placed in. We had two sized potatoes‚ 6g and 10g‚ and placed each in three different dilutions of sucrose at 0.5M‚ 0.1M‚ and 0.05M. We also placed a 6g and 10g potato piece in water to act as a control. Based on the weight percent change over 60 minutes‚ the 6g potato piece had faster water movement than the
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The movement of water down its osmotic gradient across a selectively permeable membrane is known as osmosis. The difference in the concentrations of solutes on either side of the membrane results in the establishment of an osmotic pressure. Water follows the direction of solutes‚ this means that in the event where there are more solutes outside the cell than there are inside the cell‚ water will move from the inside of the cell to the outside in order to establish water balance. When a cell is placed
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You may be wondering what diffusion‚ tonicity‚ osmosis and semipermeable membrane is. I’ll start off by explaining semipermeable membrane. A semipermeable membrane relates to the dialysis lab #1 because in dialysis tubing is considered semipermeable barriers. Semipermeable membrane is a membrane that allows passage of some particles but not others. (Lab manual) Diffusion on the other hand‚ is a form of passive transport where particles move down their concentration gradient from a region of high
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Ellice Johnson Period 1 October 12‚ 2012 Diffusion and Osmosis Shown In Solutions Section 1: Abstract This lab‚ title Diffusion and Osmosis‚ was centered around the diffusion across a cellular membrane and how exactly materials move and diffuse in concentrations. Both diffusion and osmosis are forms of movement that are part of passive transport dealing with cell membranes. Diffusion is where the solutes move from an area of high concentration to a low concentration. Water goes through the cell
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