EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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Discovery of the Structure of DNA Determining the structure for DNA was one of the outstanding science achievements of the 20th century. It started in the late 1940’s‚ when scientists were aware that DNA was most likely the molecule of life‚ even though many were sceptical since it was so "simple." They also knew that DNA included different amounts of the four bases: adenine‚ thymine‚ guanine and cytosine (usually abbreviated A‚ T‚ G and C)‚ but nobody knew what the molecule might look like. Some
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MINISTERUL EDUCATIEI‚ CERCETARII SI TINERETULUI INSPECTORATUL SCOLAR JUDETEAN DOLJ OLIMPIADA DE LIMBA ENGLEZA FAZA LOCALA -GIMNAZIU -11 APRILIE‚ 2008 Clasa aVI-a SUBJECT I Ask "wh-" questions about the underlined parts of the following sentences: 1. The English teacher gave us a test last week. 2. People often go to pubs in London because they have accessible prices. 3. The top model is wearing a silver dress tonight. 4. The weather was warm and sunny yesterday. 5. Nothing has happened
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“Wealth consists not in having great possessions‚ but in having few wants”. This great quote by the great Epictetus touches upon the materialistic values that have had been given so much importance to in this society. But when we think of wealth in general‚ the imagery that forms in our mind is not of a welfare state‚ the happy utopian world where everyone is self-sufficient; but that of expensive cars‚ solitaires and stacks of cash. But do you think that is really it? I think not. Many economists
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This clip is the 2009 opening sequence of the ITV1 show‚ Hollyoaks. The opening sequence of Hollyoaks starts with an extreme close-up (ECU) of an eye with ‘Hollyoaks’ edited in to look like it is written in the pupil. This establishes the name of the show straight away and the eye signifies to the viewer that they will be onlookers into the lives of the characters. The audience then hears the non-digetic sound of fast-paced music which sounds like that of the rock genre. It begins with a short
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The Discovery of the Fibonacci Sequence A man named Leonardo Pisano‚ who was known by his nickname‚ "Fibonacci"‚ and named the series after himself‚ first discovered the Fibonacci sequence around 1200 A.D. The Fibonacci sequence is a sequence in which each term is the sum of the 2 numbers preceding it. The first 10 Fibonacci numbers are: (1‚ 1‚ 2‚ 3‚ 5‚ 8‚ 13‚ 21‚ 34‚ 55‚ 89). These numbers are obviously recursive. Fibonacci was born around 1170 in Italy‚ and he died around 1240 in Italy
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could change everything. However‚ from my point of view‚ technology has majorly changed our life. Food has always been the most import thing as same as water to human life. If food has changed‚ people would change too. From the film called “Food Sequence”‚ I can tell there was a big change that happened to what or how we eat over the decades. Besides‚ obesity‚ GMO and food safety have always been the subject that people and scientist
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Specific Population and the Advocate Role June 23‚ 2013 Introduction “We must always take sides. Neutrality helps the oppressor‚ never the victim. Silence encourages the tormentor‚ never the tormented.” (Goodreads‚ 2013) Advocacy is about speaking up for people who cannot speak up for themselves or helping individuals speak up for themselves. An advocate is a person who is there to make sure that the best interest of the client is always kept in mind‚ advocate support their clients
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Many sequences in Chile: la memoria obstinada confront temporalities in provocative ways. Professor Ernesto Malbrán‚ who appeared in La batalla‚ reflects throughout the film on the nature of memory and argues that the dictatorship was not a definitive defeat for the left‚ but rather a temporary one. In another sequence‚ a youth band marches through the Paseo Ahumada‚ a commercial‚ pedestrian thoroughfare that symbolized Pinochet’s economic reforms of the 1980s‚ and plays “Venceremos” (“We shall Overcome”)
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groups attached to the sides. Traditionally protein is understood to assist in the development of muscle in athletic people‚ but it actually functions as structural support‚ cell adhesion‚ transport for molecules‚ and cellular communication. Through dehydration synthesis‚ the release of a water molecule from the joining of two amino acid groups‚ a peptide bond forms. For the experiment performed in lab‚ amino acids and peptide bonds found in protein were investigated in different food substances‚ it
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