LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Qualitative Analysis Lab Solubility Data Table Cations | Ag+ | Pb2+ | Cu2+ | Ni2+ | Ba2+ | NaCl | White ppt‚ AgCl(soluble in 12M HCl‚ soluble in sln of good complexing agent‚ 6M NH3) | White ppt‚ PbCl2(soluble in hot water‚ soluble in 12M HCl‚ soluble in sln of xs NaOH) | Soluble – no ppt | Soluble – no ppt | Soluble – no ppt | Na2CO3 | White ppt‚ Ag2CO3(soluble in 6M HCl‚ soluble in sln of good complexing agent) | White ppt‚ PbCO3(soluble in 6M HCl‚ soluble in sln of good complexing agent)
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Theoretical Background 4 D. Experimental Procedures 9 E. Results and Calculations 12 F. Discussion 50 G. Error Analysis 58 H. Conclusion 61 Part II: Centrifugal Pump Characteristics I. Introduction 62 J. Objectives 63 K. Theoretical Background 63 L. Experimental Procedures 68 M. Safety Precautions 68 N. Results and Calculations 69 O. Discussion 75 P. Error Analysis 79 Q. Conclusion 80 References 81 Appendix I.A Appendix II.A Appendix II.B Summary There are two parts
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The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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to titrate 25mL (±0.03mL) of unknown acid X in mL (±0.05mL) after each titre. Reading on the burette initially filled with 25mL of 0.201moldm-3 NaOH (±0.05mL) First titre 21.3 Second titre 18.2 Third titre 15.2 Fourth titre 12.0 Qualitative data Observations: When dissolving the acid X in the water‚ most of it did not dissolve and become whitish foam sitting at the top of the solution. When transferring the acid from the mortar to the flask by washing it with water‚ the low solubility
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Tori Suchy Physical Science Honors 1st block October 4‚ 2012 Balloon Powered Car Problem: In order to create a race car that can travel a minimum of eight meters powered by a nine inch balloon. We need incorporate Newton ’s Law of Motion including speed‚ velocity‚ and acceleration. Hypothesis: If a car were to be made out of homemade materials‚ then it would be possible for it to travel 8 meters powered by a nine inch balloon. Independent Variable: building the car from homemade materials
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