Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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100L = 0.007 mol (249.5896g) = 1.747g Concentration (M) Wavelength Determination (Trial One) Wavelength Determination (Trial Two) 0.13M 50%T 49%T 0.11M 55%T 54%T 0.090M 62%T 59%T 0.070M 68%T 67%T Concentration (M) Absorbance Measurements (Trial One) 2-log(%T) Absorbance Measurements (Trial Two) 2-log(%T) 0.13M 0.30M 0.30M 0.11M 0.26M 0.27M 0.090M 0.21M 0.23M 0.070M 0.17M 0.17M Unknown amount of CuSO₄: 52.0%T 2-log (%T) 2-log (52.0) =
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Mustaqim Ahmed Period 4 September 18‚ 2013 Analysis of Food Dyes in Beverages Purpose: To determine the concentration of dye in a sports drink using spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink. Materials: Beakers‚ 50-mL‚ 2-3 ; Test tubes (13 x 100)mm 3-8 ; FD&C Blue 1 stock solution‚ 50-mL ; Lens tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure:
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report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance or transmittance properties
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The Effect of Concentration on Absorbance Background Information The purpose of the “Determining Solution ‘Concentration’ Using A Spectrophotometer” lab was to use a spectrophotometer to find the relationship of concentration and absorbance obeying the Beer-Lambert law‚ which states concentration and absorbance are directly related‚ to then further determine the concentration of three unknown solutions. With the assumption that the solutions obey the Beer-Lambert law it is predicted that as concentration
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Determination of Trace Amount of Copper and Zinc in an Aqueous Solution by Atomic Absorbance Spectroscopy Abstract: The purpose of this experiment is to analyze a sample containing copper and zinc in trace amounts‚ where standard addition method was used and the instrument used is the atomic absorbance spectroscopy. The amount of copper obtained was 0.569 ppm ± 0.015 and for zinc we obtained 0.42 ppm ± 0.0027. Introduction: This method implies the excitation of particles in the solution sample
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According to the results‚ there was no significant difference between the filtered 100% water composition‚ and the filtered 90% water 10% soap composition. However‚ based on fFigure 2‚ the Average of Absorbance graph‚ showed that growth of the algae for the 100% water composition was observed to be slightly higher than the 90% water 10% soap composition. Meaning the soap may have slightly affected the growth of the algae‚ in some way. One possible‚ reason for a slightly higher growth for the filtered
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Abstract: During the lab a better agent for absorbing Procion Red Dye was trying to be found. Using the different dilutions of a stock solution and a spectrophotometer an Absorbance vs. Concentration graph was created and using the trendline from that graph it was determined which agent‚ zeolite‚ magnetic zeolite‚ or charcoal were better at absorbing the dye. From the results collected it was found that charcoal was the best at absorbing the dye‚ when moles of dye per gram of agent used were calculated
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different conditions to determine if the equilibrium constant is really constant. Fe3+ (aq) + SCN– (aq) ←→ FeSCN2+ (aq) A big part of this lab includes understanding (and in the end proving) that absorbance is directly proportional to the concentration of a solution (Beer’s Law)‚ and if Absorbance v. Concentration is graphed‚ a straight line will result. The regression line’s equation is used to determine the concentration of an unknown solution once the percent of T has been measured. The equation
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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