the Coin? RC Bauer‚ JP Birk‚ DJ Sawyer We calibrated three different molarities of copper (II) nitrate. We tested for the %Transmittance of 1M‚ 0.1M‚ and 0.01M and plotted the data collected on a calibration curve based on concentration and absorbance. We used nitric acid to dissolve a penny to produce another copper (II) nitrate to test its %Transmittance and plot that on the graph to discover the concentration of that substance which came out to be about .21M. We attempted to develop a method
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Effects of temperature manipulation and solution treatment on the Beta vulgaris craca plant cell membrane and the change of the concentration of betacyanin when placed under these various stresses Introduction: The Beta vulgaris craca plant‚ commonly referred to as the beet root contains a pigment‚ red in colour‚ called betacyanin. The betacyanin’s containment within the cells of the beet root cell relies on the stability of the plant’s membrane structure. The manipulation of the cell’s membranes
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of E. coli we distributed the bacteria into a flask of actively growing broth cultures. We kept this flask at the optimal growing conditions for E. coli to grow. We took the initial absorbance and transmittance and then allowed the E. coli to grow. Periodically we took samples of the culture and recorded the absorbance and transmittance data. We then used this data to create a graph. Using the graph we were able to determine the generation time for E. coli. Results Please refer to the worksheet
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of a solution. Results Two different graphs have been generated to represent the values gathered from the quantification of strawberry DNA. For Figure 1.‚ the relationship between the absorbance at 550nm found within each concentration of Potassium Permanganate KMNO4 “methylene blue.” The linearity for the absorbance as a function of concentration for methylene blue at 540 nm in Figure 1 is linearly applicable and its values are within close vicinity to the trend line without any dramatic linear regression
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Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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Particle in a Box Lab March 25‚ 2014 Kassandra Brady‚ Samantha Imler‚ & Michael Montone CHEM 353 Abstract: Introduction: Method I: Absorption Spectra of Conjugated Dyes This method analyzes a series of dyes with alternating double bonds. It is assumed that the π electrons are free to move and ignoring the electro static repulsion among the π electrons themselves. The theoretical model applied in this experiment is the particle in the box. The model states that a single particle moves free
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Spectrophotometry was used in the lab to determine whether non-magnetized zeolite‚ magnetized zeolite‚ or charcoal was the more effective sequestration agent for Procion Red Dye. A calibration curve was created with the known concentrations and the absorbances of the Procion Red Dye dilutions at λmax. The slope of the calibration curve was used to determine the concentration of the analytes. As a result‚ charcoal was shown to be the more effective sequestration agent. Introduction PAHs‚ polycyclic
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Yellow MTT (3-(4‚5-Dimethylthiazol-2-yl)-2‚5-diphenyltetrazolium bromide‚ a tetrazole) is reduced to purple formazan in the mitochondria of living cells. The absorbance of this colored solution can be quantified by measuring at a certain wavelength (usually between 500 and 600 nm) by a spectrophotometer. The absorption max is dependent on the solvent employed. This reduction takes place only when mitochondrial
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light-capturing pigments. Colors in the wavelengths are either absorbed or reflected by the chlorophyll in which case green is reflected and colors like red and blue are seemed to be absorbed. If blue and red wavelengths have more absorbance‚ the green doesn’t have a high absorbance. This lab is useful to help demonstrate the existence of various leaf pigments by using the process of paper chromatography.
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disrupts the membrane function in living beet cells. One aspect of this included a comparison of the amount of betacyanin that is released in different temperature treatments. A spectrophotometer will be used to measure this variable by measuring the absorbance value of the betacyanin in solution after each treatment. The results of this study will show the necessary temperature to keep the vacuole intact. It would influence common agriculture practices by indicating a more ideal environment setting and
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