I. Objective: The objective of this experiment is to determine the mass percent of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results. II. Introduction: The objective is to determine the mass percent of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results
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CHEM 163: SYNTHESIS AND ANALYSIS OF A COPPER(II) COORDINATION COMPOUND INTRODUCTION In this multi-week experiment‚ you will synthesize a compound and then analyze it to determine its empirical formula. The substance you will prepare is a vividly colored coordination compound of copper. Based on the reagents used in the synthesis procedure‚ you can assume that the final compound contains copper(II)‚ ammonia‚ sulfate‚ and water. In
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LABORATORY REPORT FOR BIO411 Experiment 5 : Cellular Respiration Experiment 6 : Photosynthesis Title: Cellular Respiration Objective: To observe and determine cellular respiration in yeast/onion cells. * Measure respiration rate using different substrates. * Measure respiration rate at different temperature. Introduction: In this laboratory experiment‚ we are given 3 task. The first one is respiration in yeast. Second is respiratory indicator and the third one is observing
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to generate a Beer’s Law plot‚ how to use elemental analysis‚ and how to form an empirical formula from the percentage of each element in the compound. Beer’s Law helps explain the relationship between absorbance and thickness off a sample‚ and accordingly‚ the relationship between absorbance and the amount of solute in a solution. Forming a Beer’s Law plot is useful when having a solution
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Abstract This experiment investigated the kinetics of the enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen
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dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration of unknown sample can then be read off a calibration curve(standard curve) created using known sugar concentrations.The dilutions of a solution of known concentration are used to determine the concentration of unknown
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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the colorless p-nitrophenylphosphate (PNPP) substrate concentration changes. We will empirically determine the concentration of production formation‚ Vmax and Km from absorbance of yellow PNP in the reaction. Results Figure 1 This figure indicates a positive linear trend for the concentration of PNP and absorbance. Table 1: Absorbance Value for Acid Phosphatase Reaction at Varying Concentrations of PNPP with the Final Product Concentration Time(s) 5mM(PNPP) Product (PNP)1 Product(PNP)1μM 2
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Typical soft drinks usually contain carbonated water‚ caffeine‚ sodium benzoate‚ flavoring agents‚ and sweeteners as well for the purpose for enhancing flavor‚ and in the case of benzoate‚ to function as a preservative. Caffeine is one of the major components that is found in several beverages like coffee‚ energy drinks‚ tea‚ and soft drinks; just to name a few. Caffeine is known to be a powerful drug‚ which many people become dependent on; when they wake up in the morning it is typical to have a
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Arrhenius equation is used to calculate the activation energy. The range in orange is between 16.5 - 37oC and the activation energy is calculated to be 9332kcal/mol. Discussion: The dilution made for all the assays was 8x which falls between an absorbance of 0.5-1.0 when measured at 405nm. On Figure. 1‚ it shows the variation of rate as a function of the weight of alkaline phosphatase. In the graph‚ the rate
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