against variables of wavelengths and light intensity. Both a control and an experimental cuvette were exposed to a range of 450 to 750nm of light and varying intensities to test for reaction rates. These effects on rate were obtained by measuring the absorbance of DCPIP on a spectrophotometer after 16 minutes. We hypothesize that the least effective wavelength will be that which is reflected back at us in broad day light‚ this being green light of about 545 nm in wavelength. In regards to intensity‚
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EVALUATION OF ANTIOXIDANT ACTIVITIES OF WHITE POPINAC Rutanachai Thaipratum Suan Sunandha Rajabhat University‚ Thailand Abstract: Free radical cause many diseases‚ for instance‚ aging‚ cancer‚ coronary heart disease‚ arthritis‚ and nervous system abnormality. Normally‚ free radical formation is controlled naturally by various beneficial compounds known as antioxidants. Antioxidants can be classified into natural and synthetic groups. Moreover‚ several analytical methods have been used for qualitative
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0.4833 x 2 = 10 x C2 0.9666 = 10 x C2 0.966610 = 0.09666g per 100ml A graph was drawn by plotting the absorbance of all the solutions measured‚ against their concentration. From this graph‚ the concentration of aspirin in the tablet solution was found to be 0.3500. This was calculated by drawing a straight line from its absorbance of 0.100 to the point where it crosses the drawn line of best fit. The point at which they met was read to be 0.3500‚ which gave the concentration
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BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
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times more abundance than DNA. However‚ what I obtained‚ the ratio of RNA: DNA is only 1.950: 4.254‚ respectively. A few possible explanations might be that RNA sample was not prepared correctly during the Orrcinol reaction procedure or that the absorbance of the RNA concentrations wasn’t taken properly‚ leading to a lower value. Another possibility might be that the RNA sample was left at room temperature for too long before the start of the procedure and so some RNA
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Why does the colour leak out of beetroot? Procedure to investigate the effect of temperature Hypothesis- The higher the temperature while cooking the beetroot‚ the more pigments well leak out from the cell membrane‚ hence the absorbance would increase. My hypothesis was concluded by the facts that beetroot contains red pigments called betalians which are located in the cell vacuole. Normally the pigments cannot leak out; however cooking the beetroot allows the movement. This is because the cell
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University Malaysia Terengganu Department of Chemical Sciences KIM3402 Laboratory Reports for Spectrometry Program: Bachelor of Science (Chemistry) Lab Group: K1 Prepared for: Dr. Noorashikin Binti MD Saleh Name of Author : Liew Ken Min Student ID : UK 31217 Signature : TABLE OF CONTENTS PAGE CHAPTER 1 Theory and Objectives of the experiment 3 1.1 General Properties of Spectroscopic Methods 1.2 UV-Vis Spectroscopy 1.3 Fluorescence Spectroscopy 1.4 Atomic Spectrometric
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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Research Question: What are the percent concentrations of Fe3+ ions by mass in fresh parsley and spinach leaves? Scientific Concept Colorimeter measures the absorbance of light by the chemical substances at a certain wavelength. By measuring the absorbance‚ the concentration of a chemical substance can be determined. For the calibration of the colorimeter‚ different concentrations of a standard solution and a control (distilled water) are prepared in cuvettes. These cuvettes are put into the colorimeter
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Equilibrium In your text (Chang‚ 6th Ed) : Ch. 15 Chemical Equilibrium‚ esp. Section 15.3 Purpose: The Law of Mass Action will be examined via a series of samples using the same reaction‚ but different stating concentrations. The equilibrium constant‚ K‚ for each reaction will be calculated‚ demonstrating that K for a given reaction at a fixed temperature is a constant‚ independent of starting concentrations. Background: For a general reaction aA + bB ↔ cC + dD‚ the Law of Mass Action
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