NOTES AND COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error
Premium Chlorophyll Spectroscopy Chromatography
beta cyanin from Beta vulgaris and various concentrations of different alcohols‚ transferred from the spot plates to the spectrometer‚ and their absorbance values our results were compared in Figure 1. The absorbance values of the solution of sodium dodecyl sulfate and beta cyanin from Beta vulgaris can be seen in Figure 2. Figure 3 shows the absorbance values of the solution of NaCL and beta cyanin from Beta
Premium Cell membrane Betalain Cell
glucose solution after being soaked in water for 5 minutes to remove any storage additives. The dialysis tube was placed within a 200mL beaker filled with distilled water and paced on a stir plate. A wavelength of 420 nm was chosen while recording the absorbance intensity of glucose‚ while a wavelength of 600 nm was chosen for starch as the respective molecules absorb most efficiently at these wavelengths. C1 is the concentration of glucose or starch in the internal solution and C2 is the concentration
Premium Glucose Starch Carbohydrate
student to test their blood glucose level and determine if they had low blood glucose‚ which indicate hypoglycemia‚ or high blood glucose‚ which indicate hyperglycemia. Introduction II. Introduction In the first part of the experiment we utilized absorbance measurements using a UV-visible light spectrophotometer to determine the concentrations of a substance. A spectrophotometer consist of a monochromator for the selection of wavelength‚ a sample holder referred to as a cuvette‚ a recorder‚ and a light
Premium Blood sugar Blood glucose monitoring Electromagnetic radiation
Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
Premium Enzyme Catalase Chemical reaction
The Hydrogen Peroxide must be added last and guaiacol should be added one tube at a time. When ready to put into the spectrophotometer the blank tubes should be put in first to blank the machine for a more accurate absorbance reading when the experimental tubes are put in. The absorbances for the experimental tubes were recorded every fifteen seconds for two minutes. This process was done for the remaining cuvettes.
Premium Enzyme Chemical reaction Chemistry
Introduction There are many different factors that can influence the rate of diffusion through a membrane. Chemical kinetics plays a large part in diffusion. In order for a solute to passively diffuse through a membrane‚ it must line up with a pore in the membrane and pass through it (textbook 101). The concentration gradient is also important for diffusion because solutes diffuse from areas of high concentration to areas of low concentration (textbook page 101). There are different factors that
Premium Osmosis Diffusion Chemistry
of 10.0 with the liberation of p-nitrophenol. The substrate is colourless‚ but the product p-nitrophenol is yellow in alkaline solution‚ absorbing maximally at 405 nrn. Thus a convenient assay for this enzyme involves monitoring the change in absorbance of the reaction medium at 405 nm. Exergonic (i.e energy producing reactions) exhibit a negative free energy change. Sometimes these reactions occur spontaneously‚ but generally some energy must be supplied to initiate the reaction; in other
Premium Chemical reaction Enzyme Chemistry
A colorimeter is a device used in colorimetry that generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. The output from a colorimeter can be shown by an analogue or digital meter and may be shown as transmittance (a linear scale from 0-100%) or as absorbance (a scale from zero to infinity). The useful range of the absorbance scale is from 0-2 but it is desirable to keep within the range 0-1 because‚ above 1‚ the results become
Premium Chemical reaction Chemistry Chlorine
Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
Premium Cellular respiration Adenosine triphosphate Citric acid cycle