Mass Determination of Ca‚ Fe and Zn in a multivitamin using Atomic Absorbance Spectroscopy (AAS) and X-Ray Fluorescence Spectroscopy (XRF) ABSTRACT: The purpose of this project was to determine the amount of calcium‚ iron and zinc present in an over the counter multivitamin. This mass was determined using atomic absorption spectroscopy (AAS) and X-ray fluorescence spectroscopy (XRF). For both analytical techniques‚ the method of standard additions was used to compensate for matrix effects.
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the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance at wavelength 436.0nm and 617.0 nm respectively
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| Bubble formation was observed as the leafs were being heated. | Table 1.1: Qualitative Data Wavelength of Incident Light (nm) | 400 | 440 | 480 | 520 | 560 | 600 | 640 | 680 | 700 | Relative Absorbance (±0.005nm) Group 1 | 0.09 | 0.11 | 0.04 | 0.01 | 0.01 | 0.01 | 0.03 | 0.03 | 0.01 | Group 2 | 0.14 | 0.15 | 0.07 | 0.02 | 0.02 | 0.02 | 0.04 | 0.04 | 0.01 | Group
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Introduction In this experiment‚ the gram negative bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients‚ the intensity of aeration‚ or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct
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MANGOSTEEN FRUIT | | | | The Mangosteen Fruit is by far the most exotic fruit in the world. It has been researched and studied so much that this site is dedicated to bring you all that information and research. | | The Mangosteen contains several classes of phytonutrient antioxidants and is the only place in nature to contain a broad spectrum of a new class of phytonutrients called Xanthones. The potent effects on human health of the Mangosteen have been observed for centuries in Southeast
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Determining absorbance of various wavelengths of light for pigments present in Coleus plants Joseph Yung (King Yung) 212831426 Adrian Ionescu Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions
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Figure 1 shows the average amount of absorbance for each tube‚ containing different levels of pH. Tube 2 had an acidic pH level‚ Tube 3 had a neutral pH level‚ and Tube 4 had a basic pH level. It is indicated that the absorbance rates were the highest for the neutral pH level‚ with a final absorbance rate of 0.166. This was followed by a basic pH (0.106). The acidic pH level had the least amount of absorbance with a final absorbance rate of 0.069. This reinforces the idea that the ALP enzyme worked
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Abstract In this experiment‚ the absorbance of KMnO4 was measured by spectrophotometric method to determine the molar concentration and the molar extinction coefficient of KMnO4. In part 1‚ in order to determine the maximum absorbance wavelength of KMnO4‚ we measured the absorbance of the sample solution which contains KMnO4 at the wavelengths between 330nm and 660nm‚ and plotted the λ and A points; the λmax was 530nm. In part 2‚ the effect of concentration on the absorbance was examined. We prepared five
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opposed to those with a lower concentration. The absorbance range for the standard curve (figure 1) was determined to be between 0.047A and 0.317A (table 1). This was determined to be the range in which the unknown absorbance had to fall. Unknown C was diluted by a factor of 1 μL UC/1001 μL dH¬¬2O and had an absorbance of .0754A. The resulting solution had a light blue coloration. The dilution was decreased to 1 μL UC/501 μL dH2O and had an absorbance of 0.089A (table 2). This solution had a slightly
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this experiment are tap water mixed with food colouring‚ Introduction: The Beer Lambert Law shows the relation between absorbance of light of an object‚ the molar absorptivity‚ the concentration of the substance‚ and the distance the light travels. The Beer Lambert Law states that there is a linear relationship between the concentration of a solution and the absorbance of said solution. If there is a linear relation‚ the resulting graph should be a straight line. If the graph is a straight line
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