The percent yield of meso-1‚2-dibromo-1‚2-diphenylethane was calculated to be 66.36% and with a melting point of 235.1°C. Therefore‚ it can be concluded that this experiment was successfully conducted as the percent yield obtained is only 33.64% off from the equilibrium point‚ and because the melting point met the literature value. The percent yield for the product was less than 100%‚ indicating that were experimental errors‚ such as an undesirable side reaction‚ or more likely‚ an incomplete reaction
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iIsolation of Chlorophylls and Beta Carotene from Plant Leaves Wang Haina A0133901R 1. Aim 1. To isolate chlorophyll and beta carotene from plant leaves using column chromatography. 2. To qualitatively characterise the pigments with UV-vis spectroscopy and TLC. 2. Results and discussion Isolation of beta carotene and chlorophylls by column chromatography Upon the loading of S1 (the extract of the plant leaves in hexane)‚ a yellow band appeared at the top part of the silica column immediately
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A colorimeter is an instrument which compares the amount of light getting through a solution (Betalain) with the amount which can get through a sample of which is clear (distilled water). A reading is then given of which shows the percentage of absorbance. This gives the results for the experiment. The independent variable for this experiment is the changing of the temperatures The dependent variable would be the colouration in the water because of the temperatures. Hypothesis Under intense
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spectrophotometer wavelength was set to 420 nm just like exercise A. After the spectrophotometer was adjusted using the blank A‚ the catechol was added to tube A. The tube was covered with paraffin and the absorption was measured within 5 seconds. The absorbance Dana Calderone readings were continued for every minute for 6 minutes. Procedures were identical for the other 3 test tubes. Table 1 Enzyme pH Blank
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should be little or no absorbance until the end point after which the absorbance should increase approximately linearly. A green filter should be used because the red permanganate solution absorbs green light. 14-4. End Point Absorbance A green filter is used because the red Fe(SCN)2+ absorbs green light. Volume SCN- 14-5. The absorbance should decrease approximately linearly with Absorbance titrant volume until the end point. After the end point the absorbance becomes independent of
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Basic Biochemistry CP2085 Effect of Temperature‚ Study and Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature
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Chemistry lab on Alcohols Cristina Tolosa Jhony Arias Data Collection: Table One: absorbance recorded with the different substances Try one Try two Substance Absorbance Substance Absorbance 4ml of water‚ 0 ml of ethanol 0.0000 4ml of water‚ 0 ml of ethanol 0.000 3ml of water‚ 1 ml of ethanol 0.125 3ml of water‚ 1 ml of ethanol 0.058 2 ml of water‚ 2ml of ethanol 0.147 2ml of water‚ 2ml of ethanol 0.087 1ml of water‚ 3ml of ethanol 0.153 1ml of water‚ 3 ml of ethanol 0.099 0 ml of
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measure enzymatic activity by the change in absorbance per second with a spectrophotometer. By testing different concentrations of peroxidase and its reaction rate in seconds‚ we were able to see that as the amount of enzyme increased the catalytic reaction also increased. The optimal amount of peroxidase concentration to be used in the subsequent experiments was determined to be 1.0 mL. Any amount above this would have caused the rate of absorbance to be too fast‚ making it too difficult to
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2. Experimental 2.1. Experimental procedure for the synthesis of bone-like CS-PEG-HA-ZrO2 nanocomposites (BNC I-III). At first‚ ZrO2 nanoparticles (NPs) were synthesized by using the previously reported method [16]. Briefly‚ aqueous solution of sodium hydroxide (purchased from HiMedia Private Ltd.) (40 ml 0.05M) was slowly added in zirconium oxychloride octahydrate (purchased from Merck India Ltd.) solution (100 ml 0.01M) prepared in methanol-water (1:1) at 5 oC temperature. The mixture was stirred
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in diVerent transfection media is not available. The only spectrophotometric assay for the quantiWcation of PEI is based on the dark blue cuprammonium complex that is formed when copper(II) ions are added to PEI [2‚3]. This complex has a strong absorbance peak at 285 nm and a weaker one at 630 nm. Unfortunately‚ the absorption maximum at 285 nm is close to that of DNA (260 nm). In addition‚ most cell culture media contain components that absorb at this wavelength. At 630 nm‚ there is much less interference
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