Melissa Cavanaugh Lab: Tuesdays at 1pm Enzyme Kinetics Lab Introduction: Enzymes are proteins that will catalyze reactions to make the rate of the reaction occur faster than it would without. It can also make the reaction occur in the first place. Tyrosinase is an enzyme that has a variety of functions and activities. It produces pigments like melanin and others that would be apparent when a fruit is cut in half and it browns. (Bien-etre 3).There is that one function that stands out and the enzyme
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Microbiology (Lab Report) Closed system growth curve Closed System Growth Curve Lab Report PURPOSE Bacteria grown in a closed system show a specific growth pattern called the growth curve which consists of four phases. The lag phase‚ which is a period of slow growth; exponential phase‚ period of maximum growth; stationary phase‚ where nutrients become the limiting factor making the growth rate equal to the death rate and the death phase where organisms die faster than they are replaced. It
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volumes that were pre-determined before the initiation of the lab.1 Placed 50uL of the enzyme in each test tube.1 Ran each reaction for the duration of five minutes.1 Quenched each test tube with 250uL of 6M NaOH after five minutes.1 Measured the absorbance of each sample at 405nm and recorded all data.1 Sodium hydroxide is corrosive and an irritant.3 Sodium phosphate is toxic and is a skin and eye irritant.4 Sodium fluoride is corrosive and is a skin and eye irritant.2 Triton X-100 is corrosive and
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The percent yield of the color Indigo that is the color of blue jeans for the three trials was determined to be 13.50%‚ 15.30% and 14.80%. A series of experiments were performed to a cloth dye comparison. The absorbance of Disulfonic Acid in the experimental trial was 608nm with an absorbance of 1.037 with Beer’s law the epsilon value was determined to be 22416.67m-1c-1. The percent yield of the second indigo Carmine experiment was determined to be 7.55%‚ 8.06%‚ 6.91%‚ 5.10% and 4.72%. Introduction:
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A. INTRODUCTION Biochemistry is the chemistry of biological systems. The practical component of biochemistry is aimed at developing your interest in and understanding modern biochemical and molecular biological experimentation. The techniques learnt in the biochemistry lab will be applicable to all life sciences. THE OBJECTIVES OF THE BIOCHEMISTRY LABORATORY INCLUDE: (1) Learning the theory behind the techniques and biochemical pathways (2) Learning the physical skills and techniques of
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A spectrophotometer can be used to analyze a solution containing anthocyanidins by locating the maximum wavelength and its corresponding absorbance value. These values can help identify which anthocyanidins are in the solution‚ as well as how much anthocyanidin‚ and by extension how much anthocyanin was present in the original
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be monitored by the spectrophotometer. This can be done by observing the colour changes over 20 minutes and record the value obtained as Vo (absorbance value). Moreover‚ a second experiment will be performed but this time it will be with the addition of the
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by adding 0.1 M HCl to basic solutions and 0.1 M NaOH to acidic solutions. It is adjusted in a way that pH 2 to 12 was equally distributed among the solutions. The absorbance of solutions 1 and 10 was measured using the spectrophotometer. The solutions were scanned from 350 nm to 800 nm at 5nm intervals. After that‚ the absorbance and wavelength of the 2 solutions was plotted in the same x-y plane using Microsoft Excel. The difference between the absorbencies of solutions 1 and 10 was also computed
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The purpose of this lab was to use a spectrophotometer to find the concentrations of glucose in varying samples of Gatorade. The varying executions included changing the temperature of reaction (room temperature instead of 40 degrees centigrade)‚ changing the time for reaction (20 minutes in the water instead of 45 minutes)‚ and changing the amount of enzyme added (0.1mL of enzyme. The results in from this lab gave data points with respect to ferricyanide. Fortunately‚ ferricyancide and glucose have
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Because the amount of light absorbed or emitted is directly proportional to the number of atoms or molecules. Raw Data and Observations: Please see attached. Results: Red #40: absorbance vs. concentration Blue # 1: absorbance vs. concentration % Composition (Grape): % Composition = (mass of Red #40 dye in sample / total mass of sample) (100%) = (4.068 x10^-4 g / 0.1019 g) (100%) = 3.992 x10^-3 (100%) = 0
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