Preparation of the propolis extracts: the propolis sample of the Brazilian native bee species Melipona quadrifasciata was obtained in May‚ 2013 in the city of Blumenau‚ SC‚ Brazil (26 ° 54’21.3 "S 49 ° 04’49.1 "W). In order to obtain a hydroalcoholic crude extract (HCE)‚ 284.3 grams of propolis were pulverized and macerated in 70% ethanol (m m-1)‚ left in a dark chamber for 7 days at room temperature‚ filtered in vacuum and taken to complete drying in a rotary evaporator with reduced pressure. In
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Enzyme assay lab report Health and safety: 4-nitrophenol is harmful. Introduction: Enzymes are quaternary structured proteins that are specific biological catalysts that speed up a reaction without being used up. They contain an active site that allows substrate to bind to a specific area on the enzyme which is of a complimentary shape of the substrate. There are two models of enzyme action‚ the Lock and Key model and the Induced Fit model. The Lock and Key model states that the enzyme has a specific
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In order to record the Absorbance Spectrum you must use the MeasureNet Spectrophotometer that is provided for you at your station. To set up the Spectrophotometer‚ first press On/Off. Then press Main Menu‚ F5 Spectroscopy‚ and F2 Absorbance(in that order). Then press Setup and F1 to set up the parameters of the scan. Set the Y axis(Intensity) maximum at 2.0 and press Enter. Leave
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Research Article Vol: 2; Issue: 1 SYNTHESIS AND ANALYSIS OF BENZOPINACOL FROM BENZOPHENONE BY PHOTOREDUCTION IN GREEN CHEMISTRY. 1 Lata.C.Potey‚ 2* Dr. Satish B. Kosalge‚ 3 Rajeshwari S. Sarode 1 2 Assistant Professor‚ Hi-Tech College of Pharmacy‚ Chandrapur. Principal‚ Hi-Tech College of Pharmacy‚ Chandrapur 3 Assistant Professor‚ Hi-Tech College of Pharmacy‚ Chandrapur. Date Received: 11 TH Jan 2014 Date of Accepted: th 16 Jan 2014 Date Published: 18th
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Relationship between Concentration of Sodium Chloride and the Rate of Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the human saliva‚ amylase breaks down starch into sugar so that large molecules
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these experiments was 2μL/mL. We then covered the tube with Parafilm and inverted it until well mixed. At this point‚ we quickly and simultaneously placed the tube in the Spec 20 and started the timer. We took an initial absorbance reading and continued to take absorbance readings every ten seconds for a total of 10 readings between 0 and 90 seconds of activity. We repeated these steps twice more with a pH 6.5 solution of the substrate. After this‚ we performed three trials each with the same
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EXPERIMENT : Iron in Breakfast Cereal by Atomic Absorption Spectroscopy OBJECTIVES : 1) To determine the actual iron content of different brands of cereals 2) To compare the experimental results with the values listed on the manufacturer’s labels. INTRODUCTION : Iron is one of the important minerals that is required for our bodies to function properly. Most of the iron in our body is found in the blood such as haemoglobin‚ approximately 60 -70% of the human body’s iron is found
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this solution 1‚ 2‚ 3‚ 4‚ 5‚ 6‚ 7‚ 8‚ 9 and 10 mL were withdrawn separately in different 10 mL volumetric flasks and volume was made up in each case up to 10mL with methanol to produce the concentrations 1‚ 2‚ 3‚ 4‚ 5‚ 6‚ 7‚ 8‚ 9 and 10 g/mL. Absorbances of these solutions were recorded at max 405.9 nm against methanol as blank using Shimadzu (1601) UV/Visible spectrophotometer‚ which is recorded in table 5.4. Standard curve is presented in figure
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this experiment we tested the absorbency of copper sulphate in different concentrations to prove the Beer Lambert Law. Introduction: This Law: Provides an experimental link between the absorbance properties of a solution of a given compound and its concentration. This is normally expressed as . A = Absorbance measured = Absorptivity C = Molar concentration L = Length of cuvette Equipment: • Gilson pipette • Eppendorf tubes • Copper sulphate • Spectrophotometer • Cuvettes • Distilled
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concentrations of BSA can be created in RIPA buffer. The known concentration’s absorbance readers can be read in a spectrophotometer‚ and graphed based concentration vs. absorbance. A standard line can be calculated and the line’s equation be interpreted (y=mx+b). Duplicate wells were used to reduce error in the standard line. The line’s equation can be used to estimate the concentration of the unknown. Substitute the absorbance of the unknown into the “y” of the equation and calculated
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