ABSTRACT: Medicinal plants are potential sources of new drugs to improve the treatment of diseases whose treatment is associated to anti-oxidative agents. In the current study‚ we carried out a comparative analysis of the antioxidant activities of extracts of the selected leaves of 6 common medicinal plants. Total Ascorbic Acid Content‚ Total Carotenoid Content‚ Antioxidant potential using FRAP assay and Total Phenol Content was quantitatively estimated from leaves of Rauvolfia serpentina‚ Cymbopogon
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aromatic residues (mainly tryptophan‚ also tyrosine). Experiments have shown that cysteine is also reactive to the reagent. Therefore‚ cysteine residues in protein probably also contribute to the absorbance seen in the Lowry Assay. [3] The concentration of the reduced Folin reagent is measured by absorbance at 750 nm.[4] As a result‚ the total concentration of protein in the sample can be deduced from the concentration of Trp and Tyr residues that reduce the Folin reagent.The method was first proposed
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0.00200 M KNCS‚ 0.00200 M Fe(NO3)3 0.200 M Fe(NO3)3‚ 0.10 M HNO3‚ 5 Cuvettes‚ 1 colorimeter 4 100ml beakers‚ 5 Test tubes 2 250ml Graduated cylinder. The first step is to calibrate the colorimeter with0.20 M Fe(NO3)3and set the absorbance at 470 nm since it is known to keep an acidic solution throughout the entirety of the experiment. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the calibration number for all of the other solutions
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this section of the practical report. Do not rewrite this section! – 5 marks * Results – This section should include: * Table of data for calibration curve * Graph – calibration curve (absorbance vs. [p-NP]. * Tables of data for enzyme assay @ 25oC and 35oC‚ showing absorbance of each timed sample & corresponding [p-NP] read from calibration curve. * Graph of [p-NP] vs. time for each temperature * Calculation of rate of reaction @ each temp. * Calculation
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Evaluation of Paracetamol Content in Tablet Dosage Form Using UV-Spectrophotometry Casilagan‚ Camela Mae C.‚ and Jimenez‚ Joshua M. Chemistry Department‚ College of Science Adamson University Ermita‚ Manila 1.0 Introduction UV-Visible Spectrophotometry is a widely-used technique in Chemical and Pharmaceutical Analysis. And this method has been very useful both for Qualitative and Quantitative Applications‚ especially for organic compounds. Some of its advantages for Quantitative purposes
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| The Chemistry of Natural Waters | Chem 111 Sec 104 | | Hyunjung Hwang | 11/6/2012 | TA: Sarah Boehm‚ Group members: Rachel Hoffman‚ Dan Hirt | Introduction Water hardness is a major part of overall water quality that affects many industrial and domestic water users. Water is considered hard when there are high concentrations of the divalent cations Magnesium and Calcium; water hardness is considered as the sum of both the calcium and magnesium concentrations and expressed as
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Interactions BigIdea 4 investigation 13 ENZYME ACTIVITY* How do abiotic or biotic factors influence the rates of enzymatic reactions? ■■BACKGROUND Enzymes speed up chemical reactions by lowering activation energy (that is‚ the energy needed for a reaction to begin). In every chemical reaction‚ the starting materials (the substrate(s) in the case of enzymes) can take many different paths to forming products. For each path‚ there is an intermediate or transitional product between reactants and
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Fenugreek (Trigonella foenum graceum L.)‚ an indigenous spice crop of India has been widely used in traditional medicine for treating various kinds of diseases. In this work‚ 10 genotypes of T. foenum-graceum were evaluated for their diosgenin content and productivity‚ ascorbic acid (AA)‚ total phenolics (TP)‚ flavonoids (TF)‚ tannin and antioxidant activity (AOX). Results indicate that fenugreek is a good source of flavonoid and total phenolics ranging from 2.74to 12.03 mg/g and 22.59 to 62.59 mg/g
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Title: Investigation of Induction Time of lac operon in E. Coli with IPTG‚ and Comparision of the Amount Beta-galactosidase produced with Lactose‚ IPTG and Antibiotic. Abstract: Introduction: An operon is a group of genes that are arranged side by side with a regulatory gene. It also includes a promoter gene‚ operator gene‚ structural genes. Regulatory genes control transcription with positive or negative signal. (Jacob‚ and Monod‚ 1961) A positive signal‚ inducer‚ would stimulate
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Mixing and Mass Energy Balance CHEM08004: Chemical Analysis and Evaluation 3/26/2014 Mass balance‚ Mixing‚ Absorbance‚ Temperature‚ Weight‚ Product Absorbance‚ Equilibrium‚ Acid‚ Alkali‚ Salt‚ pH‚ Total moles‚ Concentration‚ Potassium Permanganate‚ Ethanoic acid‚ Sodium Hydroxide‚ Sodium Chloride Introduction Mass Balance By the use of mass balances scientists can: Calculate the amounts of raw materials needed for a process‚ estimate the quantity of product that can be made
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