A wavelength of 525 nanometers is within the range for green which is the complementary color for red. The relation between absorbance and percent transmittance is inversely related and as such when absorbance is low then transmittance is high‚ and vice versa. This is given by the equation: A = 2 - log10 %T. Where A is absorbance and T is Percent Transmittance. Transmittance is the percent of light that travels through the sample. When transmittance is at its lowest‚ the corresponding
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the different amount of BSA concentrations on the x-axis as the independent variable and the absorbance measured on the y-axis as the dependent variable. The tables below
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determine the concentration of two different solutes‚ bromophenol blue and methyl orange‚ in a mixture. Material and method: Refer to practical manual page 7 Results: Part 1: Determination of Amax of bromophemol blue Table 1.1 Wavelength(nm) Absorbance 470 0.064 500 0.116 530 0.232 560 0.422 590 0.752 620 0.172 650 0.026
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hexahydrate and ethylenediamene are mixed at certain stoichiometric ratios of 0.3‚ 0.4‚ 0.5‚ 0.6‚ 0.7‚ 0.8 and 0.9 to form several complexes. Using the UV-Vis Spectrometer‚ the absorbances are taken at wavelengths 530‚ 545‚ 578‚ 622 and 640 nm due to the emergence of several complex compositions. The values of corrected absorbances and the mole fraction of en (X) are plotted to obtain a curve. From these curves‚ an intersection from the 2 trend lines are determined‚ denoting the values of x‚ needed for
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strawberry. In the experiment‚ we measured the absorbance of different concentrations of Methylene Blue. The data collected by our group is presented on Figure 1 and the average absorbance measured by whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The relationship between the concentration of Methylene Blue and the absorbance is expected to be a direct proportion.
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Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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Environmental Chemistry: Determining the Phosphate Level of Water Objective: -To understand the effects of phosphates from fertilizers into water supplies. -How to use a spectrophotometer and understand how it works. -Understand the correlation and usage of Beer’s law with calibration curves. Introduction/Procedure: Environmental protection has been a growing concern for many people in the recent years. With talks of global warming‚ deforestation‚ and rising ocean acidity in the news it is safe
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atoms in the vapour are measured by absorption or emission at characteristic wave lengths. The main usage of this was to detect one element from another in the complex sample which has taken ie.‚ it has ability to perform multiple element analyses. Absorbance should obey Beer’s law ie.‚ Analyte is measured in diluted concentrations from parts per million level to parts per trillion level and the monochromatic light radiation. There are types of spectroscopy according to the measurements we do they are
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Tan Zhi Chen 0306521 Hypothesis 1) If a calculated absorbance level is directly related to the concentration of albumin‚ then a higher concentration of albumin will lead to higher absorbance level. Results Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6
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optimum temperature and pH for the reaction of Barley alpha-amylase and starch. The predicted optimum temperature and pH is 50-60 degrees Celsius and pH of 5-6. To test this hypothesis‚ a starch solution was mixed with the amylase and then tested for absorbance‚ using a spectrophotometer‚ at several different temperatures and pHs. From these experiments it was concluded that the optimum temperature was 55 degrees Celsius and the optimum pH was 5. these results lead to the conclusion that the Barley seed
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