Chapter 2 Basis of Derivative Spectrophotometry 2.1 The Main Law of Light Absorption by a Substance Photobiological processes occur under the influence of light of ultraviolet (UV)‚ visible‚ and near infrared spectral regions. Generally‚ values of light flux intensity‚ I‚ and wavelength‚ l are used in optical measurements. The frequency index n is also considered to characterize an absorbed light. Frequency is expressed in reciprocal seconds [cÀ1] and presents itself as the ratio
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organized into the electromagnetic spectrum. Waves on the electromagnetic spectrum are measured in nanometers (nm); the shorter a wave is‚ the more energy it possesses‚ and the longer the wave the less energy it possesses (Wiles‚ 2013). The sun’s light waves are absorbed and captured by the plant’s leaves to perform Photosynthesis. Photosynthesis occurs in organelles within plant leaves called chloroplasts. Chloroplasts contain chlorophyll‚ a light absorbing pigment that in different variations absorbs
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the visible spectrum to the color violet at the other end. Then another spectrum was added called the electromagnetic spectrum (or a continuous spectrum) by James Clerk Maxwell; this included: cosmic rays‚ visible light‚ x-rays‚ and radio waves. For plants to use sunlight‚ they have to absorb it. So pigments absorb light energy. Chlorophyll is a pigment in leaves that reflect green light waves. This is the most important pigment in photosynthesis and there are also accessory pigments used in plants
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In The Great Gatsby‚ Fitzgerald creates a divide amongst the characters by separating them into different layers of society in terms of wealth. New Money is the category in which characters have previously been poor but have gradually earned vast amounts of money; old money is the situation of some characters that have always been rich through generations. Some money and no money are clear; certain characters simply have either some money or no money. Jay Gatsby falls under the category of New
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and other organisms. Light can be described in terms of light quantity and light quality. Both of these have an affect on photosynthesis. The more light a plant absorbs the more the plant is able to photosynthesize. This light is absorbed though pigments which have specific interactions with certain light waves (colors). The goal of my first experiment was to test the effect that which different colors have on photosynthesis‚ in other words the amount of oxygen that is produced. As previously mentioned
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Introduction The purpose of this experiment is to determine the maximum absorbance of fast green‚ and the chlorophylls‚ also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer. Spectroscopy is the study of light. A spectrophotometer is a machine used to determine the absorbance of light at any given wavelength. It does this by using a source of white light through a prism‚ which gives multiple wavelengths that
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enters the leaves from the earth‚ and the carbon dioxide enters from the stomata. All of these combined produce glucose and oxygen‚ which then leaves the leaf and is stored in the plant tissue. A spectrum of absorption lines or bands‚ produced when light from a hat source‚ itself produces a continuous spectrum that passes through a cooler gas. Absorption does
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This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the
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IB Home Standard Level Higher Level Options Additional Resources 8.2 Photosynthesis 8.2.1 Draw and label a diagram showing the structure of a chloroplast as seen in an electron micrograph 2D Representation 3D Representation Electron Micrograph 8.2.2 State that photosynthesis consists of the light-dependent and light-independent reactions Photosynthesis is a two-step process: 1. The light dependent reactions convert the light energy into chemical energy 2. The light
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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