University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Adol Condensation Introduction: This reaction is carried out by adding benzaldehyde and acetone into a flask. The product created is a 1‚5-diphenyl-1‚4-pentdiene-3-one‚ which includes two double bonds‚ and two benzyl ring functional groups. This is a dehydration reaction that occurs twice in order to form the diene. After obtaining the product‚ via vacuum filtration‚ it will be recrystallized and then analyzed for purity by determining both products’ melting point. The two products will be
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The Effects of Individuals’ Detection of Changes in Images on Reaction Time Abstract The purpose of this current study was to examine how individuals detected or noticed change when viewing images on their level of attentiveness. The experiment consisted of 22 participants who had to detect change across conditions for 20 minutes. These conditions were importance of change (marginal and central) and change type (color‚ location and disappearance and reappearance of images). These
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The Effect of Metal Ions on Lichens Casey Stinnett ESC 1510 October 30‚ 2014 Introduction Lichens are a sybiotic association of fungus (mycobiont) and algae (Pediastrum boryanum . The fungus engulfs the algae‚ then supplies carbs‚ nitrogen‚ and vitamins while the algae photosynthesizes. This unusual relationship enables the lichen to grow in environments where neither fungus nor algae would normally be able to solely survive. They are found on every continent‚ ranging all the way from
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Experiment Title: Torsion Vibration. Introduction : Torsion is the twisting of a metallic rod shaped object‚ when a torque is applied on two sides’ perpendicular to the radius of a uniform cross-sectional bar. Objective : Determining the natural frequency of a system undergoing tortional vibration. Theory : Using Newton’s second law of tortional system. ( [pic] …………………. ( Equation 1 ) where Io = mass moment of inertia of the disk Hence‚ [pic] ……..……
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Boys’ Code of Conduct In the article “How Boys Become Men”‚ written by John Katz‚ he analyzes situations he experienced while growing up. Katz believes that boys become men by not showing fear or feelings and not ratting on others. As in the novel‚ The Other Wes Moore‚ both Weses are forced to become men at an early age. They experience events that push them to make decisions that make them grow and think like men do. Katz details the maturation process for boys‚ as the book‚ The Other Wes Moore
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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Ravindra Nandigam‚ Ph. D.‚ South Texas College Name: Course Number: Semester: Phys 1417 Physical Science II‚ Homework #5 Instructions: 1. Type your name‚ Course number (=PHYS 1417.V01)‚ and Semester (=Summer I‚ 2013). 2. Type your answers. Include questions. 3. Make sure that all parts of each question are answered. 4. Turn in. Chapter 25 Rocks and Minerals 1. List the 8 elements which make up about 98% of Earth’s entire mass. 2. The most abundant element in the Earth’s interior is __________________
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
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