ALCIAN BLUE RATIONALE FOR USE Alcian blue is a water soluble‚ amphoteric copper phthalocyanine‚ which is most often used as a basic dye. In general‚ alcian blue has an affinity for sulfated and carboxylated groups of acid mucopolysaccharides‚ but the specific group demonstrated is dependent on the dye solution pH. RECOMMENDED FIXATIVES 10% neutral buffered formalin and Bouin’s fixative. AVOID Chromate Fixatives MODE OF ACTION (1‚ 2‚ 3) Alcian blue binds electrostatically with
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Synthesis and Purification of Acetylsalicylic Acid (ASA or Aspirin) Background Salicylic acid is a phenol as well as a carboxylic acid. It can therefore undergo two different types of esterification reactions‚ creating an ester either with the hydroxyl or with the acid. In the presence of acetic anhydride‚ acetylsalicylic acid (aspirin or ASA) is formed. Correspondingly‚ an excess of methanol will form methyl salicylate‚ which is also an analgesic. In this experiment‚ we shall use
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observed under ultraviolet light (which is discussed further down the page). An unknown substance was treated with acetic anhydrate and resulted with paracetamol. A compound that behaves in this matter is 4-aminophenol and is widespread in the industrial production of this drug. Upon these observations was the build up of “Chemical A” decided. When 4-aminophenol is t treated with acetic anhydrate it produces paracetamol. This procedure is the last stage of an industrial production of the drug called
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of the Study and the Definition of Terms. Introduction Santol or cotton fruit (Sandoricum koetjape) is a tropical fruit grown in Southeast Asia (fruitinfo.com‚ Retrieved July‚ 2014). The Santol fruit tissue is mostly sub-acid or sour in nature. When the fruit is unripe‚ this tissue is even more sour. The Filipinos like Santol even if it tastes sour and they generally eat it with some salt. Filipinos also use Santol to make marmalade. Philippine
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Abstract The purpose of this experiment is to synthesize isopentyl acetate via an esterification reaction between glacial acetic acid and isopentyl alcohol‚ using concentrated sulfuric acid as a catalyst. The product was washed using sodium bicarbonate and water‚ then dried with anhydrous sodium sulfate. It was then distilled using a standard simple distillation apparatus. The percent yield of isopentyl acetate was 60.39%. This may have been low due to incomplete distillation or evaporation once
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Science C.A.T Year 9 2014 Advanced by Sara Hamilton 9H Testing Vinegar – Acid Base Titration Aim The aim of this practical experiment was to pair up and measure the concentration of the acid in three different brands of vinegar. We added a base liquid‚ to the three different brands of vinegar until a neutralization reaction occurred. We conducted the experiment using the titration method. Hypothesis My hypothesis is that the vinegar that will have the highest acidic level will be Cornwell’s
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Lab S: Acids and Bases: Titrations | Determining Equivalence Point and Molarity Susheel Palakurthi 11661148 Partners: Justin Mwakule‚ Andrew Farrell July 29‚ 2015 CHEM 123 L02 TA: Amelia Fitzsimmons Experiment conducted: July 22‚ 2015 Introduction Acids and bases is an important aspect in chemistry. A specific example of such is in the body‚ acids and bases have to be balanced in order to provide the optimal pH in the system for normal physiological processes. Anything digested by the body has
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Introduction ESTERS are compounds derived from the reaction of a organic acid with an alcohol. Acid + Phenol/alcohol --> ester + water R--C=O + R’--OH ----> R--C=O + H2O | | C-OH C-O-R’ Esters are the compounds that give fruits their characteristic flavours and odours. ie. methyl salycilate is "Oil of Wintergreen". http://www.petrik.com/PUBLIC/library/misc/glossary_of_org_chem.htm#ESTERS Aim To synthesise the ester 3-methylbutyl ethanoate( isopentyl acetate) and to identify the product
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combination ________________________ Day 2 Materials: Part III: a penny dated after 1983 metal file or coarse sandpaper vinegar (CH3COOH) Part IV: baking soda (NaHCO3) vinegar (CH3COOH) cup spoon Procedures: Part III: Reaction between zinc and acetic acid 1. Use a file or sandpaper to completely remove the
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the components identified Materials 1. Glass Slide 2. Needle and Lancet 3. Glacial Acetic Acid 4. 0.1 NaCl 5. Bunsen Burner 6. Microscope Procedure 1. Defibrinated blood was smeared on a glass slide to form a thin film. 2. The glass slide was passed over a moderate flame back and forth until the blood was quite dry. 3. Two drops of glacial acetic acid containing 0.1 N sodium chloride was then added to the film. 4. After placing a cover slip over the
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