TEACHER NOTES & GUIDELINES Title of Lab: Effects of Temperature and Solvents on the Cell Membrane Developers of Lab: Adapted by James Kirby JD726‚ Jennifer Mortellaro JD449‚ and James Prockup JD575 from a publication by the Department of Biological Sciences at Western Michigan University‚ Kalamazoo‚ MI Overview of Lab: Description: The purpose of this lab is to illustrate the effects that temperature and solvents have on the cell membrane. Red beet tissue contains large
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The effect of temperature on the cell membranes of beetroot cells and amount of pigment released. Apparatus • Corer size 4 • White tile • A Beetroot • Automatic Water Bath • Segregated knife • A thermometer • Stopwatch Method: • First take the white tile and the corer. Then collect a cylinder of beetroot by pushing the corer into the beetroot and withdrawing it. The cylinder remains inside the corer- so push it out with the end of a pencil. • Collect 3 cylinders
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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Mayra Guadalupe October 29‚ 2012 The effect of temperature on beet cell membranes Introduction: Cell membrane is the plasma like substance that separates different organelles and molecules within an organism. The cell membranes vital function is to permit passage of protein and enzymes through their wall. Red beets‚ also known as Beta Vulgaris has a red pigment that is located in its large central vacuoles. It is my hypothesis that if the cell membrane is to experience extreme temperatures
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The effect of temperature on the permeability of cell membranes Introduction For my coursework‚ I intend to assess how temperature affects the plasma membrane of a cell. For this I will use the cell of a beetroot. Background What is a cell membrane? The cell membrane‚ also called the plasma membrane is a semipermeable lipid bilayer which is the surface of all cells that surrounds the cytoplasm. The membrane is called a bilayer as it has a double layer of phospholipids. Within the cell membrane
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To investigate the effect of variation in temperature on the permeability of cell membranes using fresh beetroot Aims In this investigation‚ you will subject fresh‚ washed beetroot discs to different temperatures to investigate the effect of temperature on the permeability of the plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly
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Pigments were first extracted from tomato paste by a 50/50 mixture of acetone/hexanes; these miscible molecules act together as one organic solvent. Pigments choose the organic layer over the tomato paste with water‚ which allowed for their extraction. K2CO3 is an ionic base that was added to deprotonate the citric acid. The ionic product of the reaction in figure 3 now prefers the aqueous layer‚ which serves to wash the pigments of the acidic impurity. Saturated NaCl pulls any water into the
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Acetone is an extremely popular solvent that is used in rubber‚ resin‚ plastics‚ lacquers‚ varnishes‚ and rubber cements. It is also used in the creation of fats‚ oils‚ and waxes where is a primary component. Acetone has many uses in modern technology including those that relate to the medical field and various domestic uses. It is a very important organic compound found in everyday life‚ but it is also a toxin and can be an extremely dangerous environmental pollutant. Acetone has many valuable
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Background of the Study Familiar household uses of acetone are as the active ingredient in nail polish removers and as paint thinner. Acetone can also be used for medical and cosmetic uses‚ such as applying acetone with alcohol for acne treatments to peel dry skin. It can also remove residues from glass and porcelain and it can also remove super glue from the skin. Perhaps‚ acetone is very useful in our everyday lives. Acetone is also known to remove ball pen marks on fabrics and clothings
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experiment is to see if we can recycle polystyrene using acetone. If this is possible‚ this can be used to help improve our environment since polystyrene itself is not biodegradable. Hypothesis: I hypothesis that polystyrene‚ mainly known as styrofoam‚ can be recycled using acetone. This cost efficient and easy method will not only motivate both individuals and industries to recycle but also improve the state of our environment. Materials: 100% acetone nail polish remover 3 small containers 4 Styrofoam
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