Chloroform‚ Acetone and Methanol) for sequential extraction. First of all plant materials were soaked in Hexane for 2-3 days with regular stirring. The mixture was then filtered by using Whatman No. 4 filter paper in to a glass beaker. 300ml of other solvent‚ Chloroform‚ was then added in the residue for 2-3 days. Similarly the mixture was filtered by using whatman No. 4 filter paper. After chloroform‚ Acetone and Methanol were added in residue for sequential extraction. Solvents; Hexane‚ Chloroform
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the cell membrane with the correct terms integral proteins‚ channel proteins and bilipid construction. Your last paragraph was very detailed with great information about the cell membrane‚ you stated “ The cell membrane is very thin and fragile‚ and it contains two different types of proteins. “Integral proteins are part of the membrane structure and cannot be removed without damaging or destroying the membrane. Peripheral proteins are bound to the inner or outer surface of the membrane and are easily
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Biological Membranes Lipid Membranes • Receptors‚ detecting the signals from outside: Light Odorant Taste Chemicals A Hormones Neurotransmitters Drugs • Channels‚ gates and pumps • Electric/chemical potential Neurophysiology Energy • Energy transduction: Photosynthesis Oxidative phosphorylation • • • • • • Structure Function Composition Physicochemical properties Self-assembly Molecular models highly selective permeability barrier Internal membranes for organelles Bilayer Permeability
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In this investigation I will be measuring the effects of temperature on the membrane permeability of beetroot. I will be measuring the amount of anthocyanin that will diffuse out of the beetroot. The way in which I will measure the anthocyanin is to check the light absorbency of the solution using a colorimeter. The higher the reading on colorimeter the more anthocyanin present in the solution To find out the permeability of the beetroot membrane I will firstly cut out cylinders of beetroot using
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Investigating the effects of temperature on cell membranes Independent variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency
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1 The Effect of Molecular Weight on the Rate of Diffusion of Substances1 Alexander Ken Libranza Group 1 Sec. A – 1L March 6‚ 2012 A scientific paper submitted in partial fulfillment of the requirements in General Biology I laboratory under Prof. Cheryl M. Talde‚ 2nd sem.‚ 2011-2012. 1 2 ABSTRACT The effect of molecular weight on the rate of diffusion was assessed using two tests: the glass tube test and the agar-water gel test. In the glass tube set-up‚ two cotton plugs soaked
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Cell Membranes and Transport Hands-On Labs‚ Inc. Version 42-0033-00-01 Exercise 1: Diffusion Observations Data Table 1: Rate of diffusion in different temperatures | | ºC | Minutes | Temperature | InitialTemp. | InitialColor | 5 | 10 | 15 | 20 | 25 | 30 | 60 | Cold | 10°celsius | clear | clear | clear | clear | clear | clear | light purple | light purple | Ambient | 25°celsius
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Lab Report Iodination of Acetone Temperature as a Variable Objective The objective of this lab is to determine the energy of activation of the iodination of acetone. This will be done by performing the reaction at differing temperatures. The same reaction orders we obtained for the previous lab will be incorporated into this experiment. The equation Ea = -8.31 x slope of ln k vs. 1/T(K) will be used to determine the energy of activation required for this this reaction. Hypothesis
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Investigation the effect of the organic solvents on the cell membrane of the beetroot Objective: To investigate the effect of different organic solvents‚ such as alcohol and paraffin oil‚ on the cell membrane of the beetroot by using the red pigments in the beetroot as indicator. Hypothesis: Organic solvents dissolve the organic matter in the cell membrane (such as phospholipids). This destroys the cell membrane‚ and the permeability of the cell membrane is disrupted casing red pigments to
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Experiment 2 : Transport Across Membrane Name : Matrix No : Group : B Semester : 1 Date of Experiment : 05.09.2013 Lecturer’s Name : Miss OBJECTIVE To study the effects of hypotonic‚ hypertonic and isotonic solutions on plant and animal cells. INTRODUCTION In cellular biology the term membrane transport refers to the collection of mechanisms that regulate the passage of solutes such as ions and small molecules through biological membranes‚ which are lipid bilayers that
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