GAS CHROMATOGRAPHY EXPERIMENT The purpose of this experiment is for the student: 1) to learn the general theoretical aspects of gas chromatography as a separation method‚ 2) to learn how to operate gas chromatographs specific to COD‚ 3) to become familiar with using the gas chromatograph (GC) to qualitatively identify components of mixtures‚ 4) to be introduced to and to interpret the quantitative data available via gas chromatography‚ 5) to gain insight into how the GC technique is used
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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because of the hydrochloric acid-catalyzed condensation reaction. This consists of one mole of acetone and two moles phenol while bubbling hydrogen chloride throughout the entire variety. In this process‚ acetone and phenol implants in a reactor with a cation exchanger. The alteration to Bisphenol A happens at around 75°C. The resulting mixture then passes in a concentrator‚ where it releases all acetone and water‚ under decreased pressure. BPA crystallizes out washed with phenol and distilled out
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Organic Chemistry I – CHEM 201-DL01 September 26‚ 2014 Organic Chemistry Experiment 4B Lab Report Extraction of Organic Compounds From Natural Sources: Extraction of Trimyristin From Nutmeg Introduction The purpose of this experiment is to illustrate how a solid natural product can be extracted from its natural source through the use of an organic solvent. Natural products are organic compounds that are synthesized by natural biological processes in plants‚ animals
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the Claisen-Schmidt reaction. Dibenzalacetone is readily prepared by condensation of acetone with two equivalents of benzaldehyde -26 -95 113.9 1. Weigh 0.212 g of pure benzaldehyde directly into a 10 x 100 mm reaction tube. 2. 2. Add 1.6 mL 95% ethanol and 2 mL of 3M sodium hydroxide solution. Then add 0.058 g of acetone to the reaction mixture. 3. Cap the tube immediately with a septum‚ and shake the mixture
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Names Period Date Understanding Chemical Hazard Labels and MSDS OBJECTIVE: To read and interpret chemical hazard labels and MSDS. MATERIALS: How to read a Chemical Label MSDS - Acetone PROCEDURE: Use the documents listed above to answer the following questions. 1. Interpret colors on a chemical hazard label. The following colors on a chemical label alert a user regarding what hazards? a. red b. yellow c. blue d. white 2. Interpret numbers on a chemical hazard label. a. A number ____ is
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of 0.1M HCl 10ml of 0.01 M HCl 10ml of 0.001 M HCl 10ml of 0.001 M NaCl 10ml 0f 0.01 M NaCl 10ml of 0.1 M NaCl 10ml of tap water 9 Test Tubes (The Effect of pH) Graduated Cylinder 6 Discs of Frozen Beetroots (3 per experiment) 10 ml of Acetone per experiment Procedure: The Effect of pH 1. Top and bottom of beetroot were cut off by scalpel. The cork borer was used to cut out about 3-4 cores‚ then each core was cut into uniform discs about 2mm thick. 2. Discs were rinsed in a small
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spread out too far to achieve accurate results. (7) In choosing a TLC solvent‚ a student found that a 50:50 hexane : acetone and 65:35 hexane : acetone give similar results. Which one should they use for their TLC plate development? Why? They should use hexane for their TLC plate development because you want to use the least polar solvent possible to achieve good results‚ and acetone is more polar than hexane. (8) Calculate the Rf values for compounds A‚ B and C below. (9) If a TLC plate shows
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LAB 1 POSTLAB REPORT (65 pts) 1. State the objective(s) of the lab. (5 pts) • To analyze the effects of specific liquids on a variety of materials • To observe potential hazards • To investigate the effects of strong bases‚ strong acids‚ acetone and bleach on a variety of materials which include sugar‚ cotton‚ nylon‚ hair‚ polystyrene‚ egg white‚ egg yolk‚ and aluminum foil. 2. Give a summary of your observations for each of the experiments. (24 pts) Sugar + H2SO4 Black clumped substance
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products in the reaction and calculate their mole ratio. II. Equipment: Balance Labeling 50 ml beaker 100 ml beaker 125 ml Erlenmeyer Flask Spatula Stirring rod Wash bottle and distilled or deionized water Wooden splint III. Reagents: Acetone‚ CH3COCH3 Copper Wire‚ Cu Nitric Acid‚ HNO3 Silver NItrate‚ AgNO3 IV. Procedure: 1. Obtain a clean and dry 50-mL beaker. Zero the balance with the beaker on the balance pan and then carefully add 1.4–1.6 g of silver nitrate to the beaker. Caution:
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