HYDROXYLATION OF PROGESTERONE BY Bacillus circulans Strain Tex 01-S3c ISOLATED FROM OIL CONTAMINATED SOIL IN OMERGA REGION‚ MAHARASHTRA‚ INDIA S. B. MALI & V.S. HAMDE Dept. of Microbiology‚ Adarsh College‚ Omerga Dist. Osmanabad‚ 413606 malisiromerga@yahoo.com and Dept. of microbiology‚ Yogeshwari College‚ ambajogai‚ Dist. Beed. Maharashtra‚ India Abstract Hydroxylations are possibly the most widespread type of steroid bioconversion. Hydroxylations can be used to buildintermediates
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Identification of Unknown Organic Compound by Melting Point (M.P)‚ Boiling Point (B.P) and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Identification of an unknown compound is important to perform through the process of melting point (M.P)‚ boiling point (B.P) and Infrared spectroscopy (I.R). Index of Hydrogen deficiency (IHD) and elemental analysis makes an experiment more efficient in
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and weighing the diacetate: (C2H4)2N2H2 + 2 CH3COOH → (C2H4)2N2H2(CH3COOH)2 piperazine piperazine diacetate In one experiment‚ 0.3126 gram of the sample was dissolved in 25 mL of acetone‚ and 1 mL of acetic acid was added. After 5 minutes‚ the precipitate was filtered‚ washed with acetone‚ dried at 110 °C and found to weigh 0.7121 gram. What is the weight percent of piperazine in the commercial material? Answer: 95.14% Sample Exercise #2: To measure the nickel content in steel
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cylinder was used to measure out 15 mL of glacial (concentrated) acetic acid. This was poured into a 125 mL Erlenmeyer flask. Both graduated cylinders were rinsed thoroughly with acetone several times. The 2‚6-dimethylaniline was added via a Pasteur pipet to the acetic acid. The graduated cylinder was rinsed with acetone and left to air dry. 2 mL of 2-chloroacetyl chloride was measured and poured into the Erlenmeyer flask. A thermometer was placed to record any temperature change during this process
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Tin container testing Following are the different tests that are performed: 1) Temper Test: Standard: IS 1993- 1982 Take the sample strip of 90mm width. Carry out the ‘zero setting’ on Erichsen Testing machine. After zero setting‚ clamp the dial by tightening the screw. Insert the sample strip in machine and lock it by test piece holder. Confirm the light arrangement and the mirror is placed on its respective position. Switch on the lamp. Rotate the back screw at uniform speed‚ so that
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testing is demonstrated the relationship between stain and stress for a certain material while it undergoes a tension force. And define the type of facture of them‚ which are two metallic‚ copper and low carbon steel and two polymeric‚ acrylic and acetone. Background Stress-strain curves show the response of material to an applied stress. It’s depend on the strain rate‚ temperature‚ and environmental conditions. They allow important information such as a material’s elastic modulus and yield stress
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compound is converted into one of a smaller molecular weight and of different physical properties‚ without changing the percentage relations of the elements composing it. The depolymerization or simply‚ the decomposition of diacetone alcohol into acetone molecules takes place via basic catalysis. The catalyst is capable of directing and accelerating thermodynamically the reaction while remaining unaltered at the end of the reaction. Diacetone alcohol is catalyzed by the hydroxide ions. The reaction
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Determining Photosynthetic Activity in Plants Using Different Wavelengths of Light and Pigmentation Abstract The roles played by light and pigmentation in photosynthesis were examined in the geranium and Coleus plant. In addition‚ the separation of certain pigments based on their molecular structure was also examined‚ with the use of paper chromatography. When different leaves of the geranium plant which were exposed to different wavelengths of light‚ photosynthesis proved to be most actively
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Discussion: The two dyes identified were disperse blue 3 and disperse red 9. Disperse red 9 separated first because it is less polar than disperse blue 3. This is due to the structure of disperse red 9 being more symmetrical than that of disperse blue 3 and having more nonpolar bonds. Disperse blue 3 is more polar because it has a hydroxide bond and has a larger dipole. The principle behind using column chromatography is that it separates compounds based on polarity. The alumina serves to allow
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Isolation and Characterization of Complex Lipids Shea Kathleen Guinto‚ Peter Julius Dale Inocando*‚ SooHyun Jung‚ Jurilla Camille Louise Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract In this experiment an egg yolk will serve as the sample to isolate lipids and separate them into two classes which is the phosphorylated lipid and the non-phosphorylated lipid. The isolated lipids are then characterized through a series of color chemical tests along with the given
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