ABSTRACT Invertase is a type of enzyme‚ a natural catalytic agent for biochemical reactions‚ can be obtained in Baker’s Yeast. Determination of the effect of pH on invertase activity is the primary objective of the experiment. Dinitrosalicyclic acid (DNS) Assay method is utilized to monitor the enzymatic activity of invertase. Invertase was subjected to different pH (3.87‚ 4.0‚ 5.5‚ 7.3 and 10.55) of buffer solution and was observed under 540 nm absorbance using spectrophotometer. After observation
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BUFFER SOLUTION (ACID) An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt. Objectives: 1. Measure the pH of the unknown solution first with indicators and then with pH paper. 2. compare the buffer solution with both a strong acid and a weak acid materials: 2 30 mL beakers 2 100mL volumetric flask 1 10mL pipet 2 aspirator 1 50mL graduated cylinder 1 10mL graduated
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equipment‚ a lab pro diagnostic tool‚ and acidic and basic solutions‚ the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid. Introduction The objective of the Irresistible lab is to determine the capacity of each solution (unbuffered and buffered both) and
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A buffer solution can maintains a narrow range of pH even when strong acid are added. In our experiment‚ we can see water is not a buffer‚ water can undergo very big changes when small amounts of strong acid or strong bases are added. When the strong acid‚ 100µl HCl was added into the dd water‚ the pH value changed from 7.38 to 5.83 which mean the dd water has turn into acid. The same phenomenon occurs while strong base‚ 100µl NaOH was added into the dd water. The pH value changed from 7.07 to 9
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Purpose: The purpose of this experiment is to analyze four drinking water samples from different places using four of the standard drinking water tests required by the EPA. The experiment will be performed by a four person group‚ with each person providing a different water sample. The four tests to be performed are: pH‚ conductivity‚ phenolphthalein and total alkalinity‚ and total and non-carbonate hardness. Data from these four tests will be gathered and analyzed‚ drawing conclusions “about
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Each group (I was with William Yung for this experiment) was tasked with testing one homogenate. The homogenate tested by our group was liquid spinach. Each team added HCl and NaOH to their homogenate. As we found out in our experiment‚ HCl was an acid so when added to the liquid spinach the pH lowered and NaOH‚ being a base‚ rose the pH level when added. The better the buffer the homogenate is‚ the less its pH changes
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Purpose This experiment shows how different concentrations of acids and bases in a solution affect its pH level. When acids and bases are combined in a solution‚ they neutralize each other. This means that depending on the concentration of both acids and bases in a solution‚ the pH will vary. A neutral pH level is 7; anything below that level is known as an acidic solution‚ while anything above is known as a basic solution. To find out the pH‚ the solutions will be diluted and tested with different
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determine the acidity of certain substances‚ we used moist and dry pH paper (litmus tests)‚ and observed the results. First we used dry pH paper to test the acidity of household ammonia‚ and it turned yellow. According to the legend‚ this indicates a weak acid. This is‚ however‚ not accurate as the vapor of the household ammonia wasn’t able to react as strongly with the pH paper since it was dry. Then‚ we used moist DI water pH paper with the same substance‚ household ammonia‚ and it almost immediately turned
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activity‚ and that catalase will denature in the pH after pH10. At the start of the reaction‚ there is no product‚ and the concentration is the same as the atmosphere. After a short time‚ oxygen accumulates at a rather constant rate. The slope of the curve at this initial time is constant and is called the initial rate. As the peroxide is destroyed‚ less of it is available to react and the O2 is produced at lower rates. When no more peroxide is left‚ O2 is no longer produced.
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2.6.6. Levels of total ammonia nitrogen‚ nitrite‚ nitrate and phosphate in the water samples The mean TAN levels in the present study in all the sampling stations were below the recommended level of 0.1 to 1.0 mg/L prescribed by Boyd (1990). However‚ when the level recommended (< 0.2 mg/L) by Chavez (2009) is taken as a standard‚ it was found to that 35% of the study period‚ TAN levels were above the acceptable limit in the feeder canal and the pond 3. Ferreira et al. (2011) has recommended 0
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