LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
of cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different
Premium Bacteria
FOOD NUTRITION BASICS LABORATORY REPORT LAB 3 PROTEINS DUE DATE Next FNB Practical NAME (CAPITALS) ______________________________GROUP LETTER___________ This work is the product of my own efforts and has not been copied from any other sources except where full acknowledgement has been given. Signed________________________________________ Student No._____________ Introduction The principle involved in this experiment is denaturation. It involves loss of ordered structure and can be caused by changes
Premium Protein Egg white Milk
MEC 325/580 Lab Report CNC Machining Lab Report Date: March 18‚ 2013 Group Leader: Jian Wu Team Members: Group Number: G4 Regment No.: D Insturctor: CONTENTS 1.Objective and introduction. 2.Principles and practice. 3.Design and result. 4.Conclusion and recommendations. Appendices 1. Objective and introduction * Objectives Operate the CNC milling machine using G-codes‚ M-codes in a CNC program. * Equipment: Minitech CNC machine (Series 2) * Machine
Premium Milling machine Numerical control
Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical
Premium Antibody Immune system Immunology
Experiment 9 and 10: Volumetric/Vinegar Analysis Abstract: The goal of the experiment that was conducted was to figure out both the molar concentration of NaOH and the standard mole ratio of the NaOH solution. In order to find the concentration of the NaOH solution‚ volumetric analysis was used. In volumetric analysis‚ a titration mechanism was utilized in order to find the reaction that the base will end up having with KHC8H4O4
Premium Sodium hydroxide Mole Concentration
Lab Report Abstract: The purpose of this lab was to see how magnesium reacts with oxygen. This reaction must be forced with heat. As magnesium changes to magnesium oxide the mass increased. Procedure: 1. Set up the stand and bunsen burne. 2. Go to crucible heat oven to obtain a crucible. 3. Weigh the crucible and lids mass. 4. Break up the magnesium ribbon on the crucible and weigh the mass of the magnesium‚ crucible‚ and lid. 5. Place the crucible on the ring stand over the flame. Allow
Free Force Mass Oxide
The different methods of measuring acids and bases in a solution By Starsky Intro to Biology September 26‚ 2011 Lab Partners: Kristen‚ Tania and Betty Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best
Premium PH
Lab Report Janice Chisholm SCI207: Dependence of Man on the Environment Instructor: Lee Ott April 28‚ 2013 1. What patterns do you observe on the information table 4? While observing the information in table 4‚ it appears that the number of fish changes from time to time and the oxygen increases and / or decreases when this occurs. 2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water?
Free Observation Scientific method Hypothesis
The objective of this lab was to. First‚ we measured out 2.00g of granular zinc and 2.03g of iodine crystals and added them together into a boiling tube‚ which we labeled "R". After‚ we added a boiling chip into a large test tube labeled "P" and we also labeled an empty regular sized test tube with a "C". When we added 5mL of acetic acid to tube R‚ the solution turned red and was hot. After we add 30 drops of the red solution into tube C and continued to swirl tube R‚ the solution became
Premium Chemistry Water Solution