EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
Premium Cell Cell membrane DNA
H83 CEL – CHEMICAL ENGINEERING LABORATORY PROJECT TITLE: SOLID LIQUID EXTRACTION OF GANODERMA LUCIDUM NAME: WIJETUNGE MUDALIGE DINITHI ISHARA WIJETUNGE ID NO: 007118 SUPERVISOR: DR SIVAKUMAR MANICKAM GROUP MEMBERS: KOW NIEN WEI AAZRA OUMAYYAH PANKAN JASON HEW YIP SENG DATE: 30 APRIL 2012 H83 CEL Chemical Engineering Laboratory Solid Liquid Extraction of Ganoderma Lucidum Author: W.M.Dinithi Ishara Wijetunge I certify that: (i) Apart from the experimental results reported in the
Premium Ultrasound
isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together with a standard caffeine. The Rf value obtained for both isolated caffeine and standard caffeine was 0.24. Keywords: solid-liquid extraction‚ multiple liquid-liquid extraction‚ sublimation‚ thin layer chromatography‚ percent caffeine
Premium Tea Solvent Caffeine
Liquid CO2 Extraction of D-Limonene This convenient‚ effective and inexpensive liquid CO2-based extraction of a natural product (D-limonene) provides an engaging example of natural products extraction using an alternative solvent (liquefied CO2). Because the procedure can be carried out in a polypropylene centrifuge tube and uses crushed dry ice as its CO2 source‚ it provides an inexpensive alternative to the equipment-intensive extraction with supercritical CO2. A short video clip is available
Premium Solvent Solubility Solution
much weaker. Besides strength of the brew‚ growing conditions‚ processing techniques- and other variables also affect caffeine content. Certain types of tea may contain somewhat more caffeine than other teas. II. Methodology Firstly‚ the extraction must be considered. Three tea bags were opened and the combined weights of the tea leaves were taken. Recording the weight is necessary. The tea leaves were returned in the bags. The next step was boiling of the tea bags in 100 mL distilled water
Premium Caffeine Solubility Solvent
Extraction of caffeine from tea bags ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer was dried
Premium Caffeine Tea
SEGi University Extraction of Benzoic Acid by Balvinder Singh (SCM 015 677) Lab Partners: 1) Surrean Rao 2) Wong Kai Jun 3) Kanaga Luckshimi 4) Sasiskala Regal ECE2332 Chemical Engineering Practices Submitted: 1st October 2013 Supervisor: Mr Ayman William 1.0 Abstract /summary The purpose of this experiment to carry out in the lab is because to determined the capability to water droplet to carry out the liquid-liquid extraction. The studies is to determine
Premium Concentration Liquid Chemistry
DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
Premium DNA Bacteria Cell
As the assistant to Michael Brune‚ the executive director of the Sierra Club‚ I am in full support of The Exploratory Fracking and Extraction act. I would like to express concerns associated with hydraulic fracturing‚ or fracking‚ on behalf of the Sierra Club. Critical problems that are commonly brought to attention are: contamination of water supplies‚ seismic activity caused by fracking‚ and it worsens the current condition of the climate. Fracking wells inject water‚ sand‚ and chemicals deep
Premium Water Petroleum Natural gas
Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
Premium Caffeine Solubility Tea