Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis
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A REPORT ON THE OVER-EXTRACTION OF GROUNDWATER IN QUEENSLAND Prepared for: THE IDEAS SUMMIT ON THE ENVIRONMENT Due: 22 May 2013 Submitted: 22 May 2013 Prepared By: Katie Gray Executive Summary The purpose of this report is to focus on an aspect of environmental economics‚ namely over-extraction of groundwater in Queensland and provide and evaluate a possible solution for this issue. Groundwater is a valuable resource which is not being protected in Queensland. Its preservation
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EXTRACTION OF CAFFEINE FROM TEA LEAVES ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer
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Extraction and Evaporation: Separating the Components of "Panacetin" Submitted- July 17‚ 2012 Introduction The purpose of this lab is to investigate the composition of a compound suspected to be Panacetin‚ a type of pain-killer. Panacetin is typically made up of sucrose‚ aspirin‚ and acetaminophen‚ but the third component in this experiment is unknown. The unknown component is suspected to be a chemical relative of acetaminophen‚ either acetanilide or phenacetin. Using techniques such as
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The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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E THICAL P RACTICES AND C OMPLIANCE WITH C ODE OF E THICS IN THE ACME L ABORATORIES L TD . ETHICAL PRACTICES AND COMPLIANCE WITH CODE OF ETHICS IN THE ACME LABORATORIES LTD. Course: Managing People at Work Course No: H501 Submitted to Mr. Neaz Ahmed Professor IBA‚ University of Dhaka Submitted by Zahra Maryam (Roll-08) Tasmima Kashem (Roll-09) A T M Shafayatul Islam (Roll-25) Institute of Business Administration‚ University of Dhaka December 31‚ 2012 Letter of Transmittal December
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nucleus of the cell‚ and that is an extraction buffer would be needed to acquire the DNA. This experiment had no control group‚ however‚ if there was a control group it might be a group with no extraction buffer. The independent variable in this experiment is the extraction buffer. The dependent variable in this experiment is the ability to extract DNA. The constants in this experiment include‚ the shape and size of the coffee filter‚ the amount of extraction buffer used‚ and the amount of isopropyl
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Introduction Extraction involves dissolving a compound or compounds either from a solid into a solvent or from a solution into another solvent. Extraction is a method used to purify a substance by removing the impurities that was added to it. A solvent- solvent extraction is a type of extraction that is most commonly done by using two substances that are typically insoluble with each other. An acid-base extraction is a solvent-solvent extraction‚ in almost every case one of the solvents is water
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Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
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