Republic of the Philippines Mindanao State University General Santos City Bio 108: Cell Biology Laboratory Report August 28‚ 2013 DNA Extraction on Onion (Allium cepa) and Human Cheek Cell Arriza‚ Rolland Merch Buscato‚ Carl G. Butil‚ Conrad G. Leonida‚ Renee Theresa ABSTRACT This activity observes the DNA present in onion (Allium cepa) and human cheek cells by extracting it with the addition of lysis buffer and chilled ethanol. The lysis buffer is prepared from squeezed onion mixed
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Chapter 19 Acme Minerals Extraction Company ACME successfully introduced teams at the Wichita plant in order to improve morale and productivity. For ACME it was important to bridge the gap between the employees working in the "brains" which worked with sophisticated technology and were highly educated‚ and those working in the "browns" which were primarily skilled and semi-skilled employees that worked in the underground extracting operations. To solve the conflicts between the two parts ACME hired
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November 5‚ 2014 Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA
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Extraction of Caffeine from Tea leaves Casinabe‚ Antonio Karlo‚*Catahay‚ Jesus Alfonso‚ Cerdena‚ O’neil‚ Cordova‚ Robelle Department of Biological Sciences‚ College of Science University of Santo Tomas‚ España Street‚ Manila 108 Group 4 2MB Date Submitted: December 13‚ 2012 ------------------------------------------------- ABSTRACT: The purpose of this experiment is to extract the amount of caffeine present in tea bags. In this experiment‚ isolation‚ characterization and purification
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1.0 TITLE Extraction of Phenol and Flavanoid compound of the edible brown seaweed species (Eucheuma Cottonii). 2.0 INTRODUCTION 2.1 An overview of Seaweed Macro-algae or “seaweeds” are multicellular plants growing in salt or fresh water. They belong to the lower plants‚ meaning that they do not have roots‚ stems and leaves. Instead they are composed of a thallus (leaf-like) and sometimes a stem and a foot (holdfast). Some species have gas-filled structures to provide buoyancy. They are often
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Discussion: Extraction and crystallization- The purpose of this lab was to extract and crystallize compounds in a mixture using acids and bases. 1 gram of the unknown was dissolved into 30 mL of ethyl acetate. The mixture was then transferred to a separatory funnel. Using aqueous solutions of different pH we were able to separate organic acids‚ bases and neutral compounds from each other. Using three 10 mL portions of HCl‚ we reacted the HCl with the base in solution. This made it possible to
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carried out in IKEA and ACME. It was interesting to know how the two extremely different company’s one a very large firm with big brand name I.e. IKEA and another one ACME very old historic British company caring the values and culture till date. I have also explain how IKEA and ACME carry out their supply chain what are the major differences operation wise within the company. It was very interesting to do the case study and it points out how the operation differences in the company actually is and how
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DNA EXTRACTION In extracting chromatin from the cells of wheat germ there are seven steps to follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes have nucleus membrane-bound organelles‚ while prokaryotic does not. The polyploidal eukaryotic cell has DNA that is held in the nucleus while the prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is more complex and extensive than the other. Prokaryote is a bacterial cell that
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Name: Leung Chun Wai Student no.: G20554292 Topic: (NT3048) Smoke extraction design for large space such as atrium 1. INTRODUCTION Atrium inside a building is an old architectural concept for about two thousand years. This concept has been developed and extended to modern buildings. Current trend of atrium design provides an ‘ideal’ external environment to the occupants with a large undivided space. The major problem for fires in atrium is hot and toxic gases accumulating and go down in the atrium
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Objective: To extract caffeine from tea powder using polar - nonpolar solvent extraction technique. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. The solution of these dissolved compounds is referred to as the Extract. Here the organic solvent dichloromethane is used to extract caffeine from an aqueous extract of tea leaves
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