Chemicals Hordenine and methyl jasmonate were purchased from Tokyo Chemical Industry (Tokyo‚ Japan). Dimethyl sulfoxide (DMSO) was obtained from Sigma-Aldrich (Tokyo‚ Japan). Plant materials Two-year-old seedlings of Vitis vinifera cv. Koshu were cultivated in perlite:vermiculite (1:1) soil in a growth chamber (11.8 Wm-2 for 14 h in a day) at 27 °C. Seedlings of Fragaria×ananassa cv. Nyoho were grown in 55% peat moss‚ 10% perlite‚ 5% vermiculite‚ and 30% decomposed granite soil at 25 ºC in a greenhouse
Premium Marketing Brand Brand management
TOPIC 1- INTRO TO CELL BIOLOGY Cell Theory: 1) all organisms consist of one or more cells 2) the cell is the basic unit of structure for all organisms (Theodor Schwann) 3) all cells arise only from pre-existing cells (i.e cell= basic unit of reproduction) (Rudolf Virchow) Cells have enormous diversity (function and size)‚ but have unity (similar basic chemistry i.e DNA‚ ATP‚ metabolism‚ etc.) ** units: 1µm= 10-6m‚ 1nm= 10-9 m (typical prokaryote is 1-5µm‚ typical eukaryote is 10-30 µm)
Premium Cell membrane Protein Signal transduction
Muscle Fibers → Endomysium → Muscle fibers → Sarcolemma → Myofibrils → Sarcomere → Actin & Mysoin myofilament ARRANGMENT OF MYOFILAMENTS A Bands Dark areas‚ w/ thick filaments I Bands Light area‚ w/ thin filaments Z Line Within the I band H Zone Light‚ Middle of each A band M Line Within H zone‚ inside A band Sarcomere Between 2 Z lines PARTS MYOSIN ACTIN THICK THIN Tail Troponin (displaces tropomyosin) Heads Actin Flexible Hinge Region Tropomyosin (covers head) MOTOR UNIT - single alpha motor
Premium Muscle
contract. The acetylcholine then combines with receptors to stimulate movement‚ or a muscle impulse. As a result‚ calcium ions flood into to the sarcoplasmic reticulum. This forms connections between the actin and myosin filaments. A cross bridge is attached to a myosin filament and when the actin filament slides opposite of the myosin‚ the cross bridge forms new links. All of this can be accomplished only if there is an energy source. The energy source for movement of a muscle contraction called
Free Action potential Muscle Acetylcholine
The Structure and Function Relationships of Proteins Proteins are a group of organic compounds whose molecules consist of carbon‚ hydrogen‚ oxygen‚ nitrogen and sometimes sulfur atoms. They are condensation polymers of amino acids that are joined by strong peptide bonds. There are 20 different types of amino acids available‚ and the different combinations of these amino acids give it different structures and specific functions to it. Below in this essay‚ will illustrate the how different structure
Premium Protein
Erythrocyte: Structure & Metabolism หัวข้อบรรยาย 1. Red cell membrane 1. Membrane lipid 2. Membrane skeleton 3. Peripheral proteins 4. Integral proteins 2. RBC metabolism 1. Glycolytic (Embden-Meyerhof) pathway 2. Hexose monophosphate shunt 3. Rapoport-Luebering pathway 2.4 Methemoglobin reductase pathwa 3. Hemoglobin 3.1 Hemoglobin structure 3.2 Hemoglobin synthesis
Premium Hemoglobin Red blood cell Protein
is rapidly conducted into the interior of the muscle fiber by structures called t-tubules. The t-tubules make contact with the Ca2+ filled sarcoplasmic reticulum. The Ca2+ released from the sarcoplasmic reticulum binds to troponin complex by the actin filaments‚ which causes the troponin complex to pull tropomyosin away. Because these chemicals have a high affinity for calcium ions they cause the myosin cross-bridges to
Premium Action potential Neuron Neurotransmitter
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
Premium Molecular biology Protein Gel electrophoresis
Skeletal System Skeletal system is the biological system providing support in living organisms. Skin‚ muscle and bones allow movement. Skin - pliable covering. Muscles do actual moving. Bones give anchor to move against. The skeleton functions not only as the support for the body but also in haematopoiesis‚ the manufacture of blood cells that takes place in bone marrow. This is why people who have cancer of the bone marrow almost always die. It is also necessary for protection of vital organs
Premium Muscle Myosin Muscular system
separate the polypeptides in the mixture and was visualized by coomassie brilliant blue and silver staining. Western blot method was used to detect the molecular weight of proteins which is selected for three different antibodies: α-β-actin targeting the α and β isoforms of actin protein‚ α-GADPH targeting Glyceraldehyde 3-phosphate dehydrogenase‚ (GAPDH); and α-BSA which targets bovine liver protein. Target protein was identified with TANDEM mass spectrometry. The experiment can be labeled a success since
Premium Gel electrophoresis Molecular biology Western blot