Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because we assume the more substrate an enzyme gets
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This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the
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Enzymes: Food & Nutrition What are enzymes Enzymes are a type of protein produced by a living organism used to catalyze chemical reactions in cells. These reactions allow the cell to build things or take things apart as needed in order to grow and reproduce. How do enzymes work - in steps 1) Substrate floats near enzyme 2) Substrate and enzyme connect – which breaks it into products 3) Products are released ex) BreadFast & Co.’s use of enzymes The company uses many different
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Advances in Data Storage Technology Contents I. Introduction 3 II. Purpose of storage 4 III. Hierarchy of storage 6 A. Primary storage 6 B. Secondary storage 7 C. Tertiary storage 7 D. Off-line storage 8 IV. Characteristics of storage 9 A. Volatility 9 B. Mutability 9 C. Accessibility 10 D. Addressability 10 E. Capacity 11 F. Performance 11 G. Energy
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Technological Advances in the Last Century Nancy September 30th‚ 2012 HUM/186 Mass Media Influences on America In the last century‚ technology has changed mass media immensely. A hundred years ago‚ people would use the post office‚ a rare telephone or word of mouth to communicate across large distances. These were not the most efficient methods of communication. However since then we have developed ways of communicating in faster‚ more effective ways. In the 1920s‚ radios started becoming
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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Biology‚ Section G 27 September 2013 Experiment 5.3 Title: The Fragility of an Enzyme Purpose: To see how easily enzyme function can be destroyed. Hypothesis: I think that the bowl with only Jell-O will set as will the bowl with heated pineapple but the Jell-O with fresh‚ uncooked pineapple will not set. Materials: Part of a fresh pineapple A blender or a fine cheese grater 3 small bowls A small box of Jell-O Pot Stove Refrigerator 2 tablespoons Procedure: 1. Cut the pineapple
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