experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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A Comparative Essay between “Disabled” and “An Unknown Girl” Moniza Alvi reflects on India as her hand is hennaed by “an unknown girl” “in the evening bazaar”. The predominantly upbeat mood of this poem contrasts with the pessimistic mood in “Disabled” written by Wilfred Owen‚ a soldier in the First World War‚ stating his anti-war view through a poem on the life of a young soldier “sewn short at elbow”‚ crippled by war. In his poem‚ the difference between past and present allows Owen to reveal
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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Submitted: October 00‚ 2014 Experiment No. 7 Unknown: 2 Compound: Table 6.1Physical Examination State Liquid Odor Smells like adhesive paste Color Pink-orange Flammability Flammable Table 6.2Physical Constant: Melting Point Parameter Experimental Theoretical (From Literature) Melting Point (oC) Trial 1 Trial 2 Average 88 88 86 86 Table 6.3Solubility Test Sample Solubility Solubility Class Water Ether 5% NaOH5% Na2CO3 5% HClConc. H2SO4 Unknown Sample + + + + + + Basic Compound Table 6
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Conclusion: (Identity‚ % error‚ other evidence‚ how to improve) The identity of Unknown A is the compound cobalt(II) chloride hexahydrate (CoCl₂•6H₂O).This is justified by the similarities in color of red-purple crystal like grains‚ which is significant in identifying the product out of the eight suggested identities. Only two had similar characteristics which were CoCl₂•6H₂O and CoSO₄•6H₂O. Before unknown A was heated‚ there was a notable common physical property of the substance being red crystal
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Purpose: The purpose of this experiment was to be able to apply our knowledge of chemistry to identify 10 unknown elements that were presented to us. We were allowed to use Bunsen burners‚ hydrochloric acid‚ use PH paper to test if the unknown solution is an acid or a base‚ and precipitate each unknown solution with each other. Procedure / Data / Conclusions: Unknown #1: I found solution 1 to be KI. While doing the precipitate reactions‚ I found that it precipitates only
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There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods. This experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by the lab instructor. The methods that
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Chemistry Lab Report Purpose The purpose of this lab is to determine the identity of an unknown liquid by measuring its density and its boiling point and try to match it with those solutions given in Table 2 of experiment 2. Procedure Part A In Part A‚ The main purpose was to find the determination of the density of the unknown (j41) and by doing that we had to determine volumes of the unknown liquid (j41) using three different volumetric devices which are graduated cylinder‚ pipette
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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