2.2 Immunoprecipitation Different protocols were compared so as to choose the one which is compatible with the characteristics of the particular protein of interest [29]. A suitable amount of nuclear extracts was diluted with 100 microliters of chilled PBS‚ 0.1% Triton 100 (to this mixture‚ the following was added to make a final concentration of 1:1000: 0.2 mM PMSF‚ 0.5 mM DTT and 15 (microgram/ microliter). It was made sure that the final concentration of nuclear extract was 0.5-1.0(microgram/
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A guide to modern methods of construction A guide to modern methods of construction December 2006 NHBC Foundation Buildmark House Chiltern Avenue Amersham Bucks HP6 5AP Tel: 01494 735394 Fax: 01494 735365 Email: info@nhbcfoundation.org Web: www.nhbcfoundation.org This guide has been written by Keith Ross‚ Paul Cartwright and Oliver Novakovic of the BRE Housing Innovation Centre © NHBC Foundation NF 1 Published by IHS BRE Press on behalf of NHBC Foundation December
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1. Introduction The waste water treatment system in Romania is characterized by a low percentage of the population connected to the saverage and waste water treatment systems. One main problem in Romania is represented by the fact that capital of Romania –Bucharest does not have a modern waste water treatment plant. The old waste water treatment plant started during the Communist period. 2. Waste water treatment in Romania. Projects in waste water sectors
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(Ancylostoma) Incidence of Hookworm Disease _ Unsanitary conditions: feces released into soil – Repeated contamination of soil – Repeated visit to same area to defecate increases transmission _ Environmental conditions: Warm and humid – Must have loose‚ aerated soil‚ with lots of humus. – Warm‚ humid climate necessary for the worm to develop in soil in shady areas _ Exposure of skin to soil – Must have access to skin so it can burrow into the skin. Pinworm (Enterobius vermicularis) _ Transmission: Eggs are
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In closing‚ Stress is a very serious matter that shouldn’t be taken lightly. As stated there’s many consequences that can follow a life full of stress‚ being life damage‚ failure‚ and even death. On the other hand there’s ways to manage and a get threw stress periods‚ also being talking to others concerning this problem. Stress is common things in the everyday life of are citizens no matter the race‚ age‚ nor place of origin. It’s a serious matter that should be taken seriously by all and handled
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TOPIC : Food Processing Industry of India B. Introduction Food processing is the conversion of raw ingredients into food items or in other forms of consumables by using different industrial processes and tools in order to produce marketable‚ consumable and long shelf life food products. Benefits of food processing are the removal of toxins‚ food preservation (longer shelf-life)‚ enabling of marketing and logistics tasks‚ and increasing of food consistency. The drawbacks in food processing
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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Phytoplankton From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search Diatoms are one of the most common types of phytoplankton. Phytoplankton are the autotrophic component of the plankton community. The name comes from the Greek words φυτόν (phyton)‚ meaning "plant"‚ and πλαγκτός (planktos)‚ meaning "wanderer" or "drifter".[1] Most phytoplankton are too small to be individually seen with the unaided eye. However‚ when present in high enough numbers‚ they may appear as a green discoloration
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Green Healthcare: Revisiting Healthcare For Sustainable Future. Abstract: Green Healthcare: Revisiting Healthcare For Sustainable Future. Keywords: Green healthcare‚ Sustainable medicine‚ Green architecture The Hippocratic oath preaches “First‚ Do no harm”. Are they not really causing inconvenience to anyone while doing good to the patients? The health care services accomplish an indebted job in treating illness. But it cannot be excluded from the list of segments that disturb the divine
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INTRODUCTION The waste produced in the course of health care activities carries a higher potential for infection and injury than any other type of waste. Therefore it is essential to have safe and reliable method for its handling. Inadequate or improper handling of biomedical waste may have serious public health consequences and a significant impact upon environment. Appropriate management of health care waste is thus a crucial component of environmental health protection and it should become an
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